摘要
实时荧光定量PCR技术是在PCR反应体系中加入荧光染料或荧光探针,利用荧光信号积累来实现实时监测整个PCR反应进程,最后通过标准曲线对未知模板进行定量分析。该技术实现了PCR从定性到定量的转变。介绍了实时荧光定量PCR技术的原理、分类及优缺点,综述了近年来实时荧光定量PCR技术在植物中的应用概况及其研究进展,旨在为该技术的进一步开发应用提供理论参考。
Real-time fluorescent quantitative PCR （RQ-PCR） is a detection method by adding fluorescent dyes or fluorescent probe into the PCR reaction system,using fluorescent signal accumulation to monitor amplification reactions of PCR reaction process,and finally the unknown template can be quantitative through the standard curve.So the detection level of PCR has improved from the qualitative to the quantitative.In order to provide theoretical reference for further application,the principle, classification,advantages and disadvantages of RQ-PCR were introduced,and its application and progress in plants in recent years were reviewed.
出处
《湖北农业科学》
2015年第13期3073-3077,共5页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(31260406)
内蒙古自然科学基金项目(2012MS0502)
关键词
实时荧光定量PCR技术
原理
内参基因
植物抗逆性
转基因产品
real-time fluorescent quantitative PCR （RQ-PCR）
principle
reference gene
stress resistance of plant
transgenicproducts
