摘要
目的探讨β-连环蛋白（β-catenin）与增殖细胞核抗原（proliferating cell nuclear antigen。PCNA）在胃癌组织中的表达，研究其与患者临床病理因素及预后的关系。方法选取2000年1月至2006年12月间126例手术获得的胃癌组织和30例正常胃组织。构建组织芯片，应用免疫组化技术检测β-catenin和PCNA在胃癌及胃正常组织的表达，分析其与胃癌患者临床病理因素及预后的关系。结果β—catenin和PCNA在胃癌组织中的阳性表达率分别为96．7％和99．2％。高于正常胃组织，差异有统计学意义（χ^2=41．082，P=0；χ^2=97．692，P=0）；应用ROC曲线检测发现PCNA对胃癌和正常胃组织的鉴别优于β-catenin，前者的灵敏度、特异度及符合率分别为84．4％、87．5％及84．6％。β-catenin与淋巴结转移有关（χ^2=6．107．P=0．04），PCNA与病理分型有关（χ^2=9．71．P=0．02）。β—catenin和PCNA在胃癌组织中的表达呈正相关（r=0．24，P=0．005）。多因素分析显示胃癌患者的TNM分期及β-catenin和PCNA均阳性表达是影响患者生存的独立因素（P=0、P=0．04），而β-catenin、PCNA的单独阳性表达并不是影响胃癌患者生存的独立因素。结论β-catenin和PCNA在胃癌组织中高表达。PCNA对胃癌和正常胃组织的鉴别优于β-catenin，联合检测两种蛋白对判断胃癌患者的预后具有一定的临床意义。
Objective To investigate the expression of β-catenin and PCNA, and its relationship with clinical parameters and prognosis. Methods Tissue microarray technology and immunohistochemical method were used to detect the expression of β-eatenin and PCNA in gastric carcinoma and normal tissue collected from January 2000 to December 2006. Data were analyzed by SPSS 19.0. Results The percentage of expression of β-eatenin and PCNA in gastric carcinoma is 96. 7%, 99. 2% ,respectively,and higher than the normal gastric tissue （χ^2 = 41. 082, P = 0 ; χ^2 = 97. 692, P = 0）. ROC curve was used to find that PCNA was better than β-catenin to discriminate gastric carcinoma from normal gastric tissue in sensitivity, specificity, coincidence rate（84. 4% ,87.5% ,84. 6% ）. The expression of [3-eatenin is related to lymph node metastasis in gastric cancer （χ^2 =6. 107,P =0. 04）. The expression of PCNA is related to pathological type （χ^2=9. 71 ,P = 0. 02）. The expression of β-catenin and PCNA in gastric carcinoma were positively correlated （r = 0. 24, P = 0. 005）. Multivariate analysis showed that TNM staging and both positive β-catenin and PCNA were independent factors impacting on the overall survival rate of patient with gastric cancer. Conclusions The expression of β-eatenin and PCNA in gastric carcinoma is signifreanfly high. PCNA is more important than β-eatenin in discriminating gastric carcinoma from normal gastric tissue. Combined detection of the two kinds of protein have some clinical significance in predicting the prognosis of patients with early gastric cancer.
出处
《中华普通外科杂志》
CSCD
北大核心
2015年第8期635-638,共4页
Chinese Journal of General Surgery
关键词
胃肿瘤
增殖细胞核抗原
Β连环素
Stomach neoplasms
Proliferating cell nuclear antigen
beta-Catenin
