摘要
在单因素试验的基础上,以皂苷浓度为指标,应用响应面法中心组合试验优化芜菁皂苷的提取条件。确定了芜菁皂苷提取最佳条件为:提取温度61.49℃,提取时间8.16 h,液料比10.50∶1.00(m L/g),甲醇浓度67.30%。在该条件下的理论值为(183.98±5.20)μg/m L与实测值(178.34±2.06)μg/m L无显著差异(P>0.05),说明回归模型拟合程度较高,可用于芜菁皂苷提取工艺的优化。
To optimize extraction process of saponins from Brassica campestris L., the central composite experiment method was used to extract saponins based on the single-factor experiments. In combination with single factor test results, the results of optimum extraction condition was as follows：extracted temperature was 61.49 ℃, extraction time was 8.16 h, ratio of liquid to material was 10.50 ∶ 1.00 mL/g, and methanol concentration was 67.30%. There wasn＇t significant difference between the predicted value （183.98±5.20）μg/mL and the actual value （178.34±2.06）μg/mL （P〉0.05）. It showed clearly that the fitting degree of regression model was well. The optimized extraction process was economical, simple, reasonable and practicable.
出处
《食品研究与开发》
CAS
北大核心
2015年第12期41-44,50,共5页
Food Research and Development
基金
新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放课题(BRYB1101)
关键词
芜菁
皂苷
提取工艺
响应面法
Saponins
extraction technology
response surface methodology
