摘要
目的研究化浊通脉方对动脉粥样硬化(AS)兔胆固醇跨膜逆转运关键蛋白小凹蛋白-1(cav-1)及亲环素A(Cyclophilin A)的影响,探讨其通过促进胆固醇跨膜逆转运抗AS的可能机制。方法 40只新西兰兔随机分为空白对照组、模型组、中药复方组、辛伐他汀组。采用单纯高脂喂养法复制兔AS模型。造模、给药15周后取胸主动脉,HE染色观察各组动脉斑块形成,蛋白印迹法(WB)测定主动脉壁内cav-1及亲环素A表达量,实时定量聚合酶链式反应(Real-time PCR)测定主动脉壁内cav-1及亲环素AmRNA表达量,观察实验药物对胆固醇逆向转运相关蛋白及mRNA表达的影响。结果 HE染色结果显示,模型组兔胸主动脉管壁上粥样斑块形成明显,各给药组较模型组有不同程度的改善。WB结果显示,与对照组相比模型组cav-1表达量明显下降(P<0.01),与模型组相比,辛伐他汀组cav-1表达增加(P<0.05),中药复方组cav-1表达量也有增加趋势,但无统计学意义;与对照组相比,模型组亲环素A表达明显增多(P<0.05),与模型组相比,中药复方组及辛伐他汀组亲环素A蛋白表达减少(P<0.01)。实时定量PCR结果显示,与对照组相比模型组cav-1 mRNA表达量明显减少(P<0.01),与模型组相比,辛伐他汀组及中药复方组cav-1mRNA表达量明显增加(P<0.01);模型组较对照组亲环素A mRNA表达量减少,但无统计学意义,辛伐他汀组及中药复方组较模型组相比亲环素A mRNA表达量明显增加(P<0.01)。结论化浊通脉方能够通过适度调节cav-1及亲环素A的表达,促进细胞内胆固醇流出,减少斑块中亲环素A的过度表达,减少AS血管壁内泡沫细胞及斑块的形成,最终达到抗AS的作用。
Objective To study the effect of Huazhuotongmai compound on the key reverse transcription protein caveolin - 1（Cav-1） and cyclophilin A of rabbits with atherosclerosis （AS） and to investigate the possible mechanism of Huazhuo Tongmai decoction （HTD） in preventing AS through promoting reverse cholesterol transport（RCT）. Methods Forty New Zealand rabbits were randomly divided into control group, model group, compound group and simvastatin group. The rabbits with AS were remodeled by feeding with simple hypercholesterol diet. The thoracic aortas were stripped for observation of artery plaque formation by HE staining after 15 weeks of drugs delivery. The Cav- 1 and cyclophilin A in aortas was tested by Western blotting （WB） and the expression of Cav 1 and cyclophilin A mRNA in aortas was tested by Real- time PCR, which were designed to observe the effects of experiment drugs on proteins related to RCT proteins. Results The results of HE staining show that there was markedly formation of atheromatous plaque on the wall of thoracic aorta, while it was improved in different degrees in medicated groups. The results of WB show that the Cav 1 in model group was significantly lower （ P 〈0. 01） compared with control group, the Cav - 1 in simvastatin group was higher （ P 〈0. 05） compared with model group,while there was no significant differences between compound groups and model group （ P 〉 0. 05）. The expression of cyclophilin A in the model group was significantly higher than that in control group （ P 〈0. 05）, and those in compound groups and simvastatin group were lower than that in model group（ P 〈0. 01）. The same results of the expression of Cav - 1 mRNA by Real - time PCR was also found, but there was no statistically significance in the expression of cyclophilin A mRNA in model group, which was lower than that in control group. The expression in compound group and simvastatin group were higher than those in model groups. Conclusion The HTD can regulate the expression of Cav 1 and cyclophilin A, promote the outflow of cholesterol in cell, reduce excessive expression of cyclophilin A and formation of foam cells and atherosclerotic plaque, finally reach the anti- atherosclerotic effect.
出处
《中西医结合心脑血管病杂志》
2015年第7期884-886,共3页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
