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基质辅助激光解析电离飞行时间质谱技术在快速鉴定真菌中的临床应用研究 被引量:12

Analysis of the clinical application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry technology in the rapid identification of pathogenic fungi
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摘要 目的总结基质辅助激光解析电离飞行时间质谱技术(MALDI-TOF MS)应用于临床真菌鉴定的特点,为评估其在真菌快速鉴定方面的重要性及临床应用价值。方法采用VITEK MS鉴定仪对2013年1月-2014年9月临床检出真菌进行鉴定,获取真菌全细胞蛋白(主要是核糖体蛋白)质谱,通过与数据库中已知菌质谱进行比较、分析,获得鉴定结果并对数据进行统计分析。结果共鉴定出真菌1 254株,质谱技术鉴定真菌共1 198株,占95.53%,未鉴定出真菌占4.47%;应用质谱技术鉴定的真菌中以假丝酵母菌属为主,共1 125株占93.91%。结论 MALDI-TOF MS技术鉴定真菌种类多,操作流程简单、快速,而且还具有很好的可重复性和准确性,是传统真菌鉴定方法的有力补充,为真菌鉴定提供了新的选择。 OBJECTIVE To summarize the characteristics of the matrix assisted laser desorption ionization time‐of‐flight mass spectrometry technology (MALDI‐TOF MS) in the quick identification of clinical pathogenic fungi so as to evaluate the clinical significance and application value of MALDI‐TOF MS in the rapid identification of fungi . METHODS The clinical isolates of fungi from Jan .2013 to Sep .2014 were identified by VITEK MS .The whole cell protein (mainly ribosomal protein) mass spectrometry of the fungi were obtained .They were compared with the fungal mass spectrometry in the database and the results were statistically analyzed .RESULTS Totally 1198 strains of fungi in the identified 1254 strains were identified by MS ,accounting for 95 .53% and others were not i‐dentified by it . Candidias dominated the majority of the fungi identified by MS with 1125 strains , holding 93 .91% .CONCLUSION MALDI‐TOF MS was a good method to identify fungi simply and quickly .The identifi‐cation spectrum of MALDI‐TOF MS was extensive .MALDI‐TOF MS also had good repeatability and accuracy .It is a powerful complement to traditional methods for identification of fungi and provides a new choice for fungal i‐dentification .
出处 《中华医院感染学杂志》 CAS CSCD 北大核心 2015年第15期3361-3363,共3页 Chinese Journal of Nosocomiology
基金 国家重大科学仪器设备开发专项(2012YQ18011708)
关键词 基质辅助激光解析电离飞行时间质谱 真菌 快速鉴定 Matrix assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS) Fungi Rapid identification
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参考文献9

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同被引文献138

  • 1徐昕荣,朱斌,马瑜璐,向弘,陈丽凤.MALDI-TOF-MS鉴定生物脱氮细菌的方法研究[J].环境科学与技术,2010,33(10):148-150. 被引量:4
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  • 3姚粟,程池,李金霞,胡海蓉.Biolog微生物自动分析系统——丝状真菌鉴定操作规程的研究[J].食品与发酵工业,2006,32(8):63-67. 被引量:15
  • 4李凤琴,吴多加,武淑真,李玉伟.基质辅助激光解吸电离飞行时间质谱检测与鉴定食品真菌的研究[J].中国食品卫生杂志,2007,19(5):385-393. 被引量:7
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