摘要
基于Tris-HCl缓冲介质中,血红蛋白对H2O2氧化L-酪氨酸体系有强烈催化作用,而Cu（Ⅱ）对这一反应体系具有强烈抑制作用,据此建立了一种酶催化荧光法测定Cu（Ⅱ）的新方法。荧光强度的变化与Cu（Ⅱ）的浓度在2.7-530μg/L范围内呈线性关系,检出限为0.6μg/L;对270μg/L Cu（Ⅱ）溶液进行11次平行测定其相对标准偏差为3.60%。该方法已成功应用于河流污水中痕量Cu（Ⅱ）的测定。
A highly sensitive and simple spectrofluorimetric method for the determination of Cu（Ⅱ）based on its fluorescence quenching effect on the hemoglobin-catalyzed reaction of H2O2 with L-tyrosine was developed.The concentration of Cu（Ⅱ）was linear with the fluorescence quenching（△F）of system under the optimal experimental conditions.The calibration curve was linear in the range from 2.7to 530μg/L with a detection limit of 0.6μg/L.The RSD for 11 replicate determinations of 270μg/L Cu（Ⅱ）was 3.60%.This method can be used for the determination of Cu（Ⅱ）in river sewage with satisfactory results.
出处
《分析科学学报》
CAS
CSCD
北大核心
2015年第4期548-550,共3页
Journal of Analytical Science
基金
河南省科技厅重点科技攻关项目(No.142102310365)
河南省教育厅科学技术重点项目(No.14A150015)
周口师范学院科研创新基金(No.zknuA201409)
