摘要
建立了一种测定菊花中7种单体酚含量的超高效液相色谱法。采用ACQUITY UPLC HSS T3色谱柱,以甲醇/乙酸铵-乙酸缓冲溶液为流动相,梯度洗脱,变波长扫描,各化合物线性较好（R2〉0.993）,在不同的添加水平都得到了较满意的回收率（于87.0%~109.0%之间）和较好的方法重复性（RSD≤6.25%）。测定了三种名菊和新疆雪菊中7种单体酚的含量,结果表明,四种菊花中含有的单体酚种类不同,含量也有差异,其中儿茶素和槲皮素的含量都很丰富。
A UPLC method was developed for determining the contents of 7 mono-phenols in chrymnflaemum. The chromatographic separation was performed on a ACQUITY UPLC HSS T3 column at 30 ℃ with methanol/ammonium acetate-acetic acid mixture as a mobile phase at a flow rate of 0.2 mL/min. The detection wavelength was variable with the change of compounds. The calibration curves of 7 mono-phenols were linear with correlation coefficients more than 0.993, and recovery （87.0%--109.0%） and accuracy （RSD ≤ 6.25%） of every compound was satisfactory. The content of 7 mono-phenols was analyzed in Xinjiang Coreopsis tinctoria and other three chrysanthemum. The results showed that different mono-phenol and unequal amount were found, and rich catechin and quercetin were detected in four chrysanthemum.
出处
《食品工业》
北大核心
2015年第8期195-197,共3页
The Food Industry
关键词
UPLC
新疆雪菊
名菊
单体酚
UPLC
Xinjiang Coreopsis tinctoria
chrysanthemum
mono-phenol
