摘要
基于棘腹蛙(Paa boulengeri)皮肤转录组数据库,釆用RT-PCR方法从棘腹蛙皮肤组织中克隆到了编码Cathelicidin-Pb前体的核苷酸序列,其完整ORF长度为441 bp,编码146个氨基酸残基。通过信号肽预测和结构域查找比对,发现Cathelicidin-Pb前体包含N端20个氨基酸残基的信号肽、101个氨基酸残基的中间间隔区(cathelin结构域)和25个氨基酸残基的成熟抗菌肽。进一步研究发现,在适度高温范围内,皮肤组织中Cathelicidin-Pb的表达随着生长环境温度的升高而逐渐增加,但是当环境温度超过27℃后,其表达量急剧降低;Cathelicidin-Pb主要在皮肤组织中表达,在肌肉组织和血液组织的表达量较低。这些结果为棘腹蛙养殖过程中疾病控制及Cathelicidin-Pb抗菌肽的后续开发提供了重要的线索。
Based on the transcriptome database of Paa boulengeri, primers were designed to clone the complete open read-ing frame （ ORF） sequence of Cathelicidin-Pb precursor gene by RT-PCR.The result showed that the ORF of Cathelicidin-Pb precursor was 441 bp and encoded 146 amino acid residues.Cathelicidin-Pb precursor consisted of three parts, inclu-ding an N-terminal signal peptide sequence of 20 amino-acid residues, a middle spacer region of 101 amino acid residues （ Cathelin domain） and a mature peptide sequence of 25 amino acid residues.Further study found that, the expression of Cathelicidin-Pb in skin tissue, increased gradually along with the increase of environmental temperature, but when the en-vironment temperature above 27 ℃, the expression amount decreased dramatically.In addition, compared with muscle and blood tissue Cathelicidin-Pb is mainly expressed in skin tissue.These results provide important clues for the disease control in Paa boulengeri breeding process and for the development of antimicrobial peptides.
出处
《淡水渔业》
CSCD
北大核心
2015年第4期26-30,共5页
Freshwater Fisheries
基金
重庆文理学院人才引进项目(R2014LX07
R2013LS13)
永川区自然科学基金项目(Ycstc
2014nc1001)
重庆市科技攻关计划(cstc2012gg-yyjs80004)
