摘要
为了揭示急性盐度胁迫下克氏原螯虾(Procambarus clarkia)肝脏抗氧化酶和鳃丝Na+/K+-ATP酶的活性变化情况,将养殖于淡水中体重为(7.51±1.23)g的克氏原螯虾幼虾直接转移至盐度4‰、8‰、12‰和16‰的水体中,于转移后6、12、24、48、96 h分别检测肝脏中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPX)、谷胱甘肽-S-转移酶(GST)以及鳃丝Na+/K+-ATP酶活性的变化。结果表明:随着盐度的升高和胁迫时间的延长,肝脏中SOD和CAT活性开始显著增强,到达一定强度后又逐渐下降。而GPX活力表现出降低的趋势,尤其在低盐度条件下,与SOD和CAT活力变化趋势相反;GST活力呈现先升高再降低的趋势;鳃丝Na+/K+-ATP酶的活力呈现出先升高然后逐渐下降的趋势,但在处理96 h后,各盐度胁迫组酶活力均高于对照组,各组之间均无显著性差异。结果说明适当升高水体盐度可以激活和增强克氏原螯虾肝脏中的抗氧化酶以及鳃丝ATP酶活力,但不同酶被激活具有一定的组织器官特异性和时序性。
In order to reveal the potential impact of acute salinity stress on the body of Procambarus clarkii, effects of low salinity stress on antioxidant enzyme activities in liver and Na＋/K＋-ATPase in gill of P.clarkii were studied.P.clarkii ju-veniles with body weight （ 7.51±1.23 ） g in freshwater were directly transferred to water with salinities at 4‰, 8‰, 12‰, and 16‰, while those in freshwater were taken as the control group.Activities of superoxide dismutase （ SOD） , catalase （ CAT） , glutathione peroxidase （ GPX） and glutathione-S-tansferase （ GST） in liver and Na＋/K＋-ATPase in gill were determined on 6, 12, 24, 48, 96 h after the transferring of P.clarkii.With the increasing of water salinity and the elongation of treated time, the liver SOD and CAT activities had a decreasing trend after an initial significant increase.The liver GPX activity decreased when the liver SOD and CAT activities were increased, especially in the lower salinity.The liver GST activity increased at first, then decreased.The Na＋/K＋-ATPase in gill also had a decreasing trend after an ini-tial increase.The activities in treatment groups were obviously higher than those in the control group after 96 hours of treat-ment, and no significant differences were found among all trial groups.The results indicated that appropriate water salinity increasing could effectively stimulate and enhance the antioxidant enzyme activities in liver and the Na＋/K＋-ATPase activi-ties in gill.However, the activitation of different enzymes had been characterized by certain specificity and time sequenti-ality.
出处
《淡水渔业》
CSCD
北大核心
2015年第4期87-91,112,共6页
Freshwater Fisheries
基金
山东省科技发展计划项目(2010GCG20901)
山东省海洋与渔业厅科技计划项目
