摘要
背景:富氢水能否用于离心运动肌损伤的防护及其相关机制尚不清楚。目的:观察富氢水对离心运动大鼠骨骼肌线粒体氧化应激及炎症反应的影响,并探讨富氢水作用的相关信号通路。方法:将40只SD大鼠随机分为对照组、离心运动组、离心运动+生理盐水组和离心运动+富氢水组。后3组大鼠连续5d进行下坡跑,跑台速度为16-18m/min,坡度-16°,90min/d,共5d。离心运动+富氢水组大鼠在每次运动后即刻腹腔注射饱和富氢水(10mL/kg);离心运动+生理盐水组大鼠注射生理盐水。结果与结论:离心运动同时给予富氢水显著上调Sirtuin-3(Sirt3)表达,提高线粒体膜电位和锰超氧化物歧化酶活性,抑制线粒体活性氧生成和线粒体DNA氧化损伤,降低炎性因子NOD样受体热蛋白结构域相关蛋白3和白细胞介素1β表达。表明富氢水除可直接清除活性氧外,还可通过上调SIRT3表达,提高线粒体能量代谢和抗氧化能力,从而抑制离心运动诱导的线粒体氧化应激和继发的炎症反应,实现骨骼肌保护效应。
BACKGROUND：It is unclear whether hydrogen-rich water can be used to protect skeletal muscle injury induced by eccentric exercise, as wel as the relative mechanism. OBJECTIVE：To observe the effect of hydrogen-rich water on the mitochondrial oxidative stress and inflammation in rat skeletal muscle after eccentric exercise, and to investigate the relative signaling pathway of hydrogen-rich water. METHODS：Forty Sprague Dawley rats were randomly divided into four groups： control group, eccentric exercise group, eccentric exercise＋saline group, and eccentric exercise＋hydrogen-rich water group. Rats in three eccentric exercise groups were exercised on a motor-driven rodent treadmil at a speed of 16-18 m/min and a slope of-16° for 90 minutes per day. Rats in the eccentric exercise＋hydrogen-rich water group were subjected to intraperitoneal injection of hydrogen-rich water （10 mL/kg） immediately after exercise; and rats in the eccentric exercise＋saline group were administrated with normal saline after exercise. Al the interventions lasted for 5 days. RESULTS AND CONCLUSION：Hydrogen-rich water intervention after eccentric exercise could markedly enhance the mitochondrial Sirtuin-3 expression, improve the mitochondrial membrane potential and activity of manganese superoxide dismutase, down-regulate the mitochondrial reactive oxygen species generation and mitochondrial DNA oxidative damage, thus inhibiting inflammatory cytokines expression, such as NLRP3 and interleukin-1β. The results indicated that hydrogen-rich saline could directly scavenge reactive oxygen species. In addition, hydrogen-rich water could improve mitochondrial energy metabolism and antioxidant capacity through up-regulation of Sirtuin-3, which in turn inhibits eccentric exercise-induced mitochondrial oxidative stress and secondary inflammation in the skeletal muscle.
出处
《中国组织工程研究》
CAS
北大核心
2015年第29期4682-4687,共6页
Chinese Journal of Tissue Engineering Research
基金
全军重点实验室开放基金(JY1406)~~
