摘要
背景:组织工程肌腱已经被应用到修复破坏的肌腱组织,作为肌腱损伤修复过程中重要的方法,已成为研究的热点。目的:通过对种子细胞的种类及优缺点、支架材料的设计及优缺点以及诱导肌腱形成的因素进行阐述,促进每个关节点的优化,有利于组织工程肌腱的成熟构建。方法:检索中国生物医学文献数据库(CBM)、中国知识资源总库(CNKI)系列数据库、中文科技期刊数据库、Pub Med数据库2000年1月至2015年1月收录的肌腱组织工程相关综述和论文报告,检索词为组织工程Tissue Engineering);肌腱(tendon);肌腱缺损,并分析其种子细胞、支架及诱导途径的研究进展。结果与结论:总结近年来组织工程肌腱在肌腱损伤中的研究工作,讨论包括种子细胞、支架、诱导因素在内的研究方法。肌腱干细胞作为种子细胞是目前组织工程肌腱研究过程中的首选,不仅具有与同种或者同体肌腱的同源性,而且分化增殖能力较强。但目前对于肌腱干细胞的获取及增殖培养无系统的方案;目前组织工程肌腱的支架材料及支架设计不能达到临床上对于组织工程肌腱力学的要求,形成的肌腱组织力学性能差,与宿主组织融合差,后期容易退化甚至功能性废用等原因;诱导因素作为最后的关键因素,对于其诱导因子的选取和利用是调控肌腱组织发育的必备条件。但诱导因子的种类与利用途径之间的联系及相互关系尚不完全明确,也有待进一步发展。
BACKGROUND：Tissue-engineered tendons have been used to repair the damaged tendon tissue. Use of tissue-engineered tendons for repair of tendon injury has become a hot spot in this research field. OBJECTIVE： To elaborate the types, advantages and disadvantages of seed cels, the design method, advantages and disadvantages of scaffold materials, and the factors that induced the formation of tendon, so as to promote the optimization of each joint, al of which benefit for mature construction of tissue-engineered tendons. METHODS： The related reviews and paper reports of tendon tissue engineering published from January 2000 to January 2015 were retrieved from Chinese Biomedical Literature Database （CBM）, China Knowledge Resources Database （CNKI） series database, Chinese Citation Database and PubMed database. The key words were “tissue engineering; tendon; tendon defect”. The research progress of seed cels, scaffold material and induction factors were analyzed. RESULTS AND COMCLUSION：The recent research of tissue-engineered tendons for repair of tendon injury has been summarized. Seed cels, scaffold, induction factors were discussed. Tendon stem cels, as a kind of seed cels, are currently the first choice in the process of tissue engineering tendon research, because tendon stem cels have the homology of the homogenous or autologous tendons and possess strong differentiation and proliferation capacities. However, there have been no systematic schemes regarding acquisition and proliferation and culture of tendon stem cels. The currently designed tissue-engineered tendons cannot meet the clinical requirements because of poor mechanical properties of tendon tissue, poor integration with the host tissue, being susceptible to degradation in late period and functional disuse. Induction factors are the laft key factors for tissue-engineered tendons for repair of tendon injury. The selection and use of induction factors are prerequisites for the regulation of tendon tissue development. But the categories of induction factors and the association and interrelationship between induction factors have not been fuly clear and studies are needed to further investigate these uncertainties.
出处
《中国组织工程研究》
CAS
北大核心
2015年第29期4710-4714,共5页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金应急管理项目(81441117)~~
