摘要
为筛选芥蓝×甘蓝型油菜种间杂交后代的遗传背景,加速回交转育进程,采用蕾期授粉结合胚挽救手段进行远缘杂交,获得芥蓝和甘蓝型油菜的种间杂种F1和BC1群体。利用已有的220对SSR引物对双亲进行多态性筛选,获得多态性引物51对。挑选均匀分布在甘蓝9条染色体上、扩增稳定、条带清晰的33对多态性SSR引物,对3株F1单株和35株BC1单株进行遗传背景筛选。NTSYSpc2.11a分析结果表明：F1植株的遗传背景与亲本甘蓝型油菜更为接近,遗传相似系数为0.74;而BC1植株的遗传背景差异较大,与亲本芥蓝的遗传相似系数在0.26~0.65之间。在35株BC1植株中,单株14Y1与芥蓝的遗传背景最为相近,形态观察结果进一步验证了该单株的遗传背景与回交亲本芥蓝更相似,可用于下一步回交转育。
To screen the genetic background and increase the back-cross breeding efficiency of the interspecific hybrids and their back-cross offsprings between Chinese kale （Brassica oleracea var. alboglabra, CC, 2n=2x=18 ） and rapeseed （Brassica napus, AACC ）, the F1 inter-specific hybrids and their BC1 backcross offsprings between Ogu-CMS Chinese kale and rapaseed were obtained through hand pollination combined with embryo rescue technology. A total of 220 SSR primers were used to detect polymorphisms between the parents, in which 51 pairs of SSR primers showed polymorphism. 33 pairs of primers were selected to analyze the genetic background of 3 F1 plants and 35 BC1 plants, with even distribution on 9 chromosomes and stable amplifications. The results of analysis by software NTSYSpc2.1 la showed that the similarity coefficients between FL plants and rapeseed was 0.74, which indicated that the genetic background of F1 plants was closer to rapeseed than to Chinese kale, while the similarity coefficients between BC1 plants and Chinese kale varied from 0.26-0.65, with big genetic background differences among BC1 plants. In all 35 BE1 plants, the genetic background of individual 14Y1 was the closest to Chinese kale, which was further confirmed by morphological observation. Thus, the individual 14Y1 could be used as donor plant for further backcross.
出处
《中国蔬菜》
北大核心
2015年第8期14-21,共8页
China Vegetables
基金
国家"863"计划项目(2012AA100202)
创新能力专项资金项目(2014EG134236)
农业部大宗蔬菜产业技术体系项目(CARS-25)
农业部园艺作物遗传改良重点开放实验室项目
关键词
芥蓝
甘蓝型油菜
种间杂种
遗传背景
SSR标记
Chinese kale
Rapeseed
Inter-specific hybrid
Genetic background
SSR markers
