摘要
目的：建立抗病毒咀嚼片的质量标准。方法：采用薄层色谱法对抗病毒咀嚼片中连翘、知母、广藿香进行定性鉴别,采用高效液相色谱法测定连翘中连翘苷的含量。色谱柱为Diamonsil C18,流动相为乙腈-水（20∶80,V/V）,流速为1.0 ml/min,检测波长为230 nm,柱温为25℃,进样量为10μl。结果：薄层色谱图中,供试品在与对照品相应位置上显相同颜色的斑点。连翘苷进样量在0.053 5~2.675μg范围内与其峰面积呈良好的线性关系（r=0.999 9）;精密度、稳定性、重现性试验的RSD≤1.74%;平均加样回收率为98.94%,RSD为1.84%（n=6）。结论：该方法操作简便、快速,结果准确、可靠,重复性好,可用于抗病毒咀嚼片的质量控制。
OBJECTIVE： To establish the quality standard for Anti-virus chewable tablet. METHODS： TLC was used for the qualitative identification of Forsythia suspensa, Anemarrhena asphodeloides and Pogostemon cablin in Anti-virus chewable tablet, and HPLC was conducted to determine the content of forsythin in E suspensa. The column was Diamonsil C18 with mobile phase of acetonitrile - water （20 ： 80, V/V） at the flow rate of 1.0 ml/min, the detection wavelength was 230 nm, column temperature was 25 ℃, and injection volume was 10 μl. RESULTS： The test sample and the reference sample displayed same color spots on the corresponding position in TLC diagram. The linear range of forsythin was 0.053 5-2.675 pg （r=0.999 9）; RSDs of precision, stability and reproducibility tests were no more than 1.74 % ; average recovery was 98.94 % （RSD = 1.84%, n = 6）. CONCLUSIONS： The method is simple, rapid, accurate, reliable and reproducible, and can be used for quality control of Anti-virus chewable tablet.
出处
《中国药房》
CAS
北大核心
2015年第24期3420-3422,共3页
China Pharmacy
基金
贵州省中药现代化专项计划立项项目(No.黔科合中药字〔2013〕5028号)
