摘要
背景:脂肪干细胞具有来源广泛、含量丰富、容易获取,培养条件简单且扩增能力强等优点,有可能成为继骨髓间充质干细胞之后最具前景的骨组织工程种子细胞。目的:探讨兔脂肪干细胞与脱钙骨支架材料复合修复尺骨缺损的可行性。方法:构建兔尺骨缺损动物模型,将单纯脱钙骨材料植入右侧缺损区,设为对照组,将成骨诱导后的兔脂肪干细胞-脱钙骨支架材料复合物植入左侧缺损区,设为实验组。植入后12周,获取骨缺损处组织标本进行CT扫描和组织学检测。结果与结论:经CT扫描,实验组骨缺损断端与材料的接合部位已不能清晰分辨,断端外侧可观察到平行骨痂。对照组脱钙骨未完全降解,可观察到清晰的骨断端,未观察到连续骨痂,骨缺损处大多为纤维连接,未出现骨性修复。经组织学检测,实验组缺损处为典型再生骨组织,存在骨细胞和骨陷窝以及骨小梁结构,骨细胞和骨陷窝数量较多,仅有部分骨小梁结构形成,并有少量胶原样结构穿插于再生骨组织之间;对照组有脱钙骨基质残留现象,并存在部分胶原纤维样组织,在边缘可观察到一定的骨膜成骨反应,但程度较轻,且未出现大片再生骨样组织。以上结果表明兔脂肪干细胞成骨诱导后与脱钙骨支架材料复合可以用于修复尺骨缺损。
BACKGROUND：Adipose-derived stem cel s have a wide variety of sources and strong proliferation ability, which are easy to access and simple to culture. Therefore, adipose-derived stem cel s that are secondary to bone marrow mesenchymal stem cel s are expected to become the most promising seed cel s for bone tissue engineering. OBJECTIVE：To explore the feasibility of rabbit adipose-derived stem cel s with demineralized bone matrix to repair ulna defects. METHODS：Ulna defect model was made in rabbits. Demineralized bone matrix was implanted into the right defect region as control group. After osteogenic induction, rabbit adipose-derived stem cel s/demineralized bone matrix composite was implanted into the left defect region as experimental group. At 12 weeks after implantation, defect tissues were taken for CT scanning and histological detection. RESULTS AND CONCLUSION：CT results showed that there was unclear boundary between the broken ends of fractured bone and the composite material in the experimental group, and paral el cal uses out of the broken end could be seen. In the control group, the broken end was clearly seen and no cal us occurred continuously. Fibers were connected at the defect site, and no new bone occurred. Histological findings showed that typical regenerated bone tissues were seen in the experimental group with osteocytes, bone lacunae and bonetrabeculae;there were more osteocytes and bone lacunae, but bone trabecula was only seen in a part of bone defects;a few of col agens interlarded the regenerated bone tissues. In the control group, the residual of demineralized bone matrix was seen as wel as some col agenous fibers, and periosteal bone formed a little, but no large amount of regenerated osteoid tissues were found. These findings indicate that under osteogenic induction, rabbit adipose-derived stem cel s combined with demineralized bone matrix are feasible to repair ulna defects.
出处
《中国组织工程研究》
CAS
北大核心
2015年第28期4520-4525,共6页
Chinese Journal of Tissue Engineering Research
基金
四川医科大学自然科学基金(2013ZRQN071)~~
