摘要
目的对B(A)血型进行血清学和分子生物学定型,并对B(A)血型患者的血样进行分析,研究此类血型患者的输血策略。方法对58例正反定型不符的医院送检样本和本中心的献血员血样采用试管法鉴定ABO血型,同时采用PCR-SSP方法进行ABO基因分型;采用盐水介质和Liss-coombs卡交叉配血,分析论证38例该血型患者输注B型红细胞的可行性。结果 58例血样经血清学和分子生物学定型为B(A),其中39例为B(A)04,19例为B(A)02。38例临床医院送检的B(A)病例,与随机选择的100位B型献血员交叉配血,除1例有高效价抗-A的献血员与2例患者次侧盐水介质W+外,其余均相合。结论 B(A)血型有明确的血清学特异性,分子生物学定型B(A)02和B(A)04较常见,B(A)患者优先考虑输注O型洗涤红细胞和AB型血浆、AB型血小板,亦可输注配血相合的B型红细胞,抗-A效价低于128的B型血浆和B型血小板也可考虑输注。
Objective To test the serological-typing and geno-typing for people with blood type B（A） and study clinical transfusion strategy for patients with blood type B（A）.Methods ABO Serological-typing was tested using tube method and geno- typing was detected using PCR- SSP in 58 cases,in which 38 cases were patients and 20 cases were blood donors.Salt method and Liss-Coombs gel card method were used for cross-match typing.Results All 58 case samples were B（A）.Thirty-nine cases were B（A）04 and 19 cases were B（A）02.Thirty-eight samples of patients were crossmatched with 100 samples from blood donors,except 1 case who was a blood donor with high titeranti-A and was incompatible with 2 case patients in salt method,while other cases were compatible.Conclusion B（A） phenotype has unique serological characteristics.B（A）02 and B（A）04 are main genotypes in Chinese.It is recommended that blood transfusion for B（A） patients should be type O washed red blood and AB plasma and platelet.B group red blood cell can be transfused to B（A） patient.If anti-A titer is lower than 128,group B plasma and platelet can also be transfued.
出处
《北京医学》
CAS
2015年第8期795-797,共3页
Beijing Medical Journal
