摘要
饲养并繁殖酸敏感离子通道1(ASIC1)基因敲除杂合子小鼠,提取小鼠尾部组织DNA,采用聚合酶链反应(PCR)方法鉴定子代小鼠基因型。ASIC1基因敲除小鼠的繁育和鉴定均获得成功,子代小鼠基因型分别为杂合子(ASIC1+/-)、纯合子(ASIC1-/-)和野生型(ASIC1+/+)。
To breed and identify acid sensing ion channel 1 （ ASIC1 ） gene knockout mice, so as to lay the founda- tion for studying ASIC1 protein. The heterozygote mice were bred and reproduced. Genome DNA extracted from the murine tail was subjected to PCR test for genotype identification. Breeding and reproducing of ASIC1 knockout mice were both successful, and the genotypes of the offspring mice were heterozygous （ASIC1 ＋/- ） , homozygous （ASIC1 -/- ） ,and wild-type（ASIC1 ＋/＋ ）. Appropriate methods of breeding,reproducing and identifying can effective- ly obtain ASIC 1 - / - mice
出处
《安徽医科大学学报》
CAS
北大核心
2015年第9期1341-1343,共3页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81271949)
