摘要
目的通过对2012年深圳市鼻病毒基因特性及分子变异的分析，初步了解深圳市鼻病毒流行及基因变异情况。方法荧光RT-PCR对门诊采集的流感样病例样本进行筛查，通过巢式RT-PCR对其VP4～VP2片段和VP1基因进行扩增，利用ClustalW和MEGA等软件进行分子变异分析。结果2012年深圳市有A、B两个型别的鼻病毒同时流行，其中A型占多数，包括A47、A31、A90、A18等亚型，其中出现了两株A47和A31的基因重组病毒；VP1分子的变异具有型间差异，且变异位点散乱；不同亚型的受体结合区（BC、DE和Ⅲ环）位点并不一致，具有多态性；25个药敏位点中有5个位点（1121V、L123M、V167I、Y189H、H259G）出现了变异。结论多个亚型的鼻病毒在深圳存在广泛的流行，而且仍在不断的发生重组和变异。
Objective To analyze the genetic characteristics and molecular variation of human rhi- novirus strains isolated in Shenzhen. Methods RNA samples were extracted from nasopharyngeal swab samples collected from influenza-like subjects in Shenzhen and analyzed by fluorescent RT-PCR. The VP4- VP2 and VP1 gene regions of human rhinovirus strains were amplified by nested RT-PCR. Clustal W and MEGA programs were used to evaluate molecular variation of the human rhinovirus strains. Results Both human rhinovirus A and B were prevalent in Shenzhen during 2012. Human rhinoviruses A was the predomi- nant pathogen, including subtypes A47, A31, A90, A18 and so on. Two recombinant strains of human rhi- noviruses A47 and A31 were detected. The mutations scattered on the VP1 protein and varied in different subtypes. The receptor binding sites （loop BC, DE and HI） in different subtypes showed polymorphism. Five out of twenty-five drug sensitivity sites （I121V, L123M, V167I, Y189H and H259G） showed muta- tion. Conclusion Multiple subtypes of human rhinovirus were prevalent in Shenzhen and were in a state of constant recombination and variation.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2015年第7期527-531,共5页
Chinese Journal of Microbiology and Immunology
关键词
鼻病毒
基因重组
分子变异
Human rhinovirus
Gene recombination
Molecular variation
