摘要
目的:探讨复方血竭制剂对远端型溃疡性结肠炎(ulcerative colitis,UC)小鼠模型肺与肠的保护作用.方法:60只♂BALB/c小鼠随机分成4组(n=15),正常组、葡聚糖硫酸钠(dextran sulfate sodium,DSS)组、复方血竭制剂组、DSS+复方血竭制剂组.全部小鼠适应环境喂养7 d,第8天,正常组、DSS组小鼠予以生理盐水1 mL灌胃,复方血竭制剂组、DSS+复方血竭制剂组小鼠予以复方血竭制剂[0.075 g/(kg·d)]1 mL灌胃,每只小鼠自由觅食,继续喂养10 d.通过疾病活动指数(disease activity index,DAI)评分、结肠组织病理评分(histopathology score,HI)、肺HI评分、苏木精-伊红(hematoxylin-eosin,HE)染色评价炎症严重程度.通过酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)、免疫组织化学、实时荧光定量PCR(real-time quantitative PCR,RT-QPCR)测定结肠与肺组织中白介素(interleukin,IL)-6炎性因子的表达量.结果:与正常组、复方血竭制剂组比较,DSS组、DSS+复方血竭制剂组小鼠一般情况、DAI评分、结肠HI评分、肺HI评分均升高(P<0.05);与DSS+复方血竭制剂组比较,DSS组小鼠结肠与肺组织炎症程度更重(P<0.05);且小鼠肺与结肠组织中IL-6表达量显著升高(P<0.05).结论:复方血竭制剂可以下调炎性因子IL-6表达量,缓解小鼠实验性UC及UC肺损伤.
AIM： To investigate the protective effect of compound dracorhodin enema on the lung and intestine of mice with distal ulcerative colitis （UC）. METHODS： Sixty male BALB/c mice were randomly divided into four groups （n = 15 each）： a normal group, a dextran sulfate sodium （DSS） group, a compound dracorhodin enema group, and a DSS ＋ compound dracorhodin enema group. After inducing UC, the normal group and DSS group were given I mL saline, and the other two groups were given compound dracorhodin enema [0.075 g/（kg · d）, 1 mL] by gavage. After treatment, disease activity index （DAI） score, colonic histopathology score （HI）, lung HI score, and hematoxylin-eosin （HE） staining were used to evaluate the severity of inflammation. Enzyme-linked immunosorbent assay （ELISA）, immunohistochemistry, and real- time quantitative PCR （RT-QFCR） assay were used to detect the expression of interleukin （IL）-6 in the lung and colon. RESULTS： Compared with the normal group and compound dracorhodin enema group, DAI score, colonic HI score, and lung HI score were significantly higher in the other two groups （P 〈 0.05）. Compared with the DSS ＋ compounddracorhodin enema group, colonic and pulmonary tissue inflammation was significantly more severe and pulmonary and colonic expression of IL-6 was significantly higher in the DSS group （P 〈 0.05）. CONCLUSION： Compound dracorhodin enema can decrease the expression of IL-6 and therefore alleviate UC and UC related lung injury in mice.
出处
《世界华人消化杂志》
CAS
2015年第21期3352-3360,共9页
World Chinese Journal of Digestology
关键词
复方血竭
溃疡性结肠炎
炎性因子
白介素-6
肺
Compound dracorhodin
Ulcerativecolitis
Inflammatory factor
Interleukin-6
Lung
