摘要
目的：探讨血清及脑脊液硫化氢（H2S）、一氧化氮（NO）水平检测在中枢神经系统感染（CNSI）患儿诊断和鉴别中的临床作用。方法：随机选取2012年11月~2014年10月于我院进行医治的85例中枢神经系统感染患儿作为观察组,其中病毒性脑炎患儿38例（病脑组）、结核性脑膜炎患儿20例（结脑组）、化脓性脑膜炎27例（化脑组）;随机选取25例非中枢神经系统感染患儿作为对照组。采用双抗体夹心ELISA法和ELISA酶联免疫法检测以上中枢神经系统感染患儿血清及脑脊液中H2S、NO水平,并与对照组患儿进行分析比较,同时比较不同类型感染患儿血清及脑脊液中H2S、NO变化水平。结果：观察组患儿血清中H2S水平比较：化脑组最高,结脑组次之,均显著高于对照组（均P〈0.01）;血清中NO水平比较：结脑组最高,化脑组次之,病脑组最低,均显著高于对照组（均P〈0.01）;脑脊液中H2S水平比较：化脑组最高,结脑组次之,均显著高于对照组（均P〈0.01）;脑脊液中NO水平比较：结脑组最高,化脑组次之,病脑组最低,均显著高于对照组（均P〈0.01）。结论：H2S、NO水平增高与中枢神经系统感染的发生与发展紧密相关,三类中枢神经系统感染患儿血清及脑脊液中H2S、NO水平差异显著,血清及脑脊液H2S、NO水平检测对于中枢神经系统感染患儿诊断和鉴别具有重要价值。
Objective：To explore value of determination of level of H2S、NO in serum and cerebral spinal fluid for diagnosis and differentiation diagnosis of central nervous system infection in children.Methods：A total of 85 children with infection of the central nervous system treated in our hospital from November,2012 to October,2014 were randomly chosen as the observation group,including 38 viral encephalitis cases,20 tubercular meningitis cases and 27 purulent meningitis cases.Besides,25 children without CNS disease were randomly chosen as control group.Then level of H2S、NO in in serum and cerebral spinal fluid of all children were determined with double antibody sandwich method and enzyme-linked immunosorbent assay,and were compared between the differenet groups.Results：The level of H2 Sin serum of the observation group：level in children with purulent meningitis was the highest,followed by children with tubercular meningitis,which were both obviously higher than that of the control group（t=3.092,P〈0.01,t=8.951,P〈0.01）.The level of NO in serum：serum level in children with purulent meningitis was the highest,followed by children with tubercular meningitis and viral encephalitis,which were both obviously higher than that of the control group（t=3.092,P〈0.01,t=8.951,P〈0.01,t=9.132,P〈0.01）.The level of H2 Sin cerebral spinal fluid：H2Slevel in children with purulent meningitis was the highest,followed by children with tubercular meningitis,which were obviously higher than that of the control group（t=3.291,P〈0.01,t=6.934,P〈0.01）.Level of NO in cerebral spinal fluid：it was the highest in children with purulent meningitis,followed by children with tubercular meningitis and viral encephalitis,which were both obviously higher than that of the control group（t=14.91,P〈0.01,t=2.769,P〈0.01,t=3.132,P〈0.01）.Conclusion：Increased level of H2 S,NO may be closely correlated with the pathogenesis and development of infection of the central nervous system.The determination of level of H2S、NO in serum and cerebral spinal fluid was meaningful in the diagnosis and differentiation diagnosis of central nervous system infection in children.
出处
《海南医学院学报》
CAS
2015年第9期1305-1308,共4页
Journal of Hainan Medical University
基金
湖北省科技厅(2012-cbd0121)~~
