摘要
以竹根姜幼苗为材料,采用室内水培试验和茎基部注射接种姜青枯菌方法,探讨外源茉莉酸甲酯(MeJA)诱导竹根姜产生姜瘟病抗病性的生理生化机制。结果显示:外源MeJA处理可以显著降低竹根姜的病情指数,提高对姜瘟病的抗性,而对青枯菌的致病力没有直接的抑制作用。MeJA处理可促进竹根姜内源H2O2在前期迅速积累,同时诱导增强姜块内苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)、过氧化氢酶(CAT)等防御酶以及病程相关蛋白几丁质酶和β-1,3-葡聚糖酶的活性,促进姜块内木质素含量上升。研究表明,MeJA能通过调控竹根姜细胞内下游信号分子H2O2的水平来提高防御相关酶的活性和病程相关蛋白的表达,促进木质素的合成,提高了竹根姜的抗病性。
With‘Zhugenjiang’ ginger （Zingiber officinale Roscoe） as the object of study,we analyzed the physiological and biochemical mechanism of MeJA induced to the disease resistance.The research discovered that the treatment of exogenous Methyl jasmonate（MeJA） could significantly reduce disease indices of ‘Zhugenjiang’ ginger,and increase resistance against Bacterial Wilt of Ginger.no directly inhibitory effect was detected on pathogenicity of Ralstonia solanacearum in vitro.MeJA-treated ‘Zhugenjiang’ ginger could speedly promote endogenous H2O2 content during the early stage,and simultaneously induce the increases of activities of phenylalanine ammonialyase（PAL）,peroxidase（POD）,polyphenol oxidase（PPO）,catalase（CAT）,chitinase and β-1,3-glucanase associated with increase of lignin content.The results exhibited that MeJA regulated the downstream signaling molecule H2O2 level to enhance activities of enzymes related to defense and expressions of pathogenesis-related proteins（PR proteins） in ginger cells,resulting in promoting lignin accumulation and furthermore improving disease resistance in ginger.
出处
《西北植物学报》
CAS
CSCD
北大核心
2015年第7期1415-1420,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
重庆市自然科学基金(cstc2012jjA80012)
重庆市教委科学技术研究项目(KJ131112)
重庆三峡学院院级重点项目
关键词
竹根姜
茉莉酸甲酯
过氧化氢
病程相关蛋白
木质素
＇ Zhugenjiang＇ ginger（Zingiber officinale Roscoe）
methyl jasmonate
hydrogen peroxide
pathogenesis-related proteins
lignin
