摘要
目的：研究丹参注射液对5/6肾切除致肾功能炎性损伤模型大鼠的保护作用。方法：将50只大鼠随机均分为假手术（等体积蒸馏水）组、模型（等体积蒸馏水）组、阳性对照[卡托普利5 mg/（kg·d）]组和丹参注射液低、高剂量[1.6、4.8 ml/（kg·d）]组。除假手术组外,其余各组大鼠采用5/6肾切除方法复制模型,第6周起连续给药4周。每周检测各组大鼠24 h尿蛋白,末次给药后测定大鼠血清中肌酐、尿素氮、白细胞介素1β（IL-1β）、IL-2和IL-10的含量,观察大鼠肾组织病理学变化和巨噬细胞CD68浸润程度。结果：与假手术组比较,模型组大鼠给药1-4周时24 h尿蛋白增加,肾组织病理变化明显,给药后血清中肌酐、尿素氮、IL-1β、IL-2、IL-10含量和CD68阳性表达均增加;与模型组比较,阳性对照组和丹参注射液低、高剂量组大鼠给药2-4周时24 h尿蛋白降低,肾组织病理变化改善,血清中肌酐、尿素氮、IL-1β含量和CD68阳性表达均降低,IL-10含量增加,IL-2含量仅阳性对照组大鼠降低,以上差异均具有统计学意义（P〈0.01或P〈0.05）。结论：丹参注射液可改善5/6肾切除模型大鼠肾组织病理变化、减轻炎性损伤。
OBJECTIVE：To study the protective effects of Danshen injection on 5/6 nephrectomiy induced model rats with inflammatory renal injury. METHODS：50 rats were equally randomized into a sham-operation（isometric distilled water）group,a model（isometric distilled water） group,a positive control [captopril 5 mg/（kg·d）] group and Danshen injection low-dose and high-dose [1.6,4.8 ml/（kg·d）] groups. Models were established by performing 5/6 nephrectomy on the rats in all groups except the sham-operation group. The rats were given drugs for 4 consecutive weeks from the 6th week. 24 h urine protein was determined for all rats every week. After the last administration,the contents of creatinine,urea nitrogen and interleukins 1 β（IL-1 β）,IL-2 and IL-10 in rats＇ serum were determined. Pathological changes and infiltration of macrophages CD68 in rats＇ kidney tissues were observed. RESULTS：Compared to the sham-operation group,those in the model group had more 24 h urine protein and more obvious renal pathologic changes during 1-4 weeks of administration,and higher contents of creatinine,urea nitrogen,IL-1β,IL-2 and IL-10 and CD68 positive expression in serum. Compared to the model group,during 2-4 weeks of administration,those in the positive control group and Danshen injection low-dose and high-dose groups had less 24 h urine protein,milder renal pathologic changes,and lower contents of creatinine,urea nitrogen,IL-1 β and CD68 positive expression in serum,and higher content of IL-10,and only the rats in the positive control group demonstrated lower IL-2 content. There of the above were statistical differences（P〈0.01 or P〈0.05）. CONCLUSIONS：Danshen injection can alleviate renal pathologic changes and reduce inflammatory injury of the kidneys of 5/6 nephrectomiy induced model rats.
出处
《中国药房》
CAS
北大核心
2015年第25期3518-3521,共4页
China Pharmacy
关键词
丹参注射液
5/6肾切除
炎性损伤
肾功能
大鼠
Danshen injection
5/6 nephrectomy
Inflammatory injury
Renal function
Rat
