摘要
目的建立水果中4-氯苯氧乙酸、氯吡脲和2,4-二氯苯氧乙酸3种植物激素残留量的超高效液相色谱测定方法。方法样品经乙腈匀浆提取,经氨基固相萃取小柱净化后,采用Waters ACQUITY UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7μm),以乙腈-0.1%磷酸为流动相,梯度洗脱,流速:0.2 ml/min,柱温:35℃,检测波长为230 nm,外标法峰面积定量。结果 4-氯苯氧乙酸、氯吡脲、2,4-二氯苯氧乙酸在1μg/ml^100μg/ml时与峰面积呈良好的线性关系,相关系数为0.999 5~0.999 8,平均回收率为75%~97%,相对标准偏差为0.5%~1.1%,检出限为0.10 mg/kg^0.20 mg/kg。结论该法简单快速灵敏度高,适用于日常水果的检测。
Objective To establish an ultra performance liquid chromatography( UPLC) method for the determination of 3 plant growth regulator residuals in fruits,such as 4- chlorophenoxyacetic acid,forchlorfenuron,2,4- dichlorophenoxyacetic acid.Methods The sample was homogenated with acetonitrile,purified by NH2 solid phase extraction column,separated on Waters ACQUITY UPLC BEH C18( 2. 1 mm × 100 mm,1. 7 μm) column for gradient elution using a mobile phase of acetonitrile and0. 1% phosphoric acid at flow rate of 0. 20 ml / min and the column temperature at 35 ℃,then detected at 230 nm,and quantified with external standard method. Results The calibration curves showed a good linearity with correlation coefficients from0. 999 5 to 0. 999 8 when the linear range was among 1 μg / ml- 100 μg / ml for 4- chlorophenoxyacetic acid,forchlorfenuron,and 2,4- dichlorophenoxyacetic acid. The average recovery rate ranged from 75% to 97% with the relative standard deviation of 0. 5%- 1. 1%. The detection limit was among 0. 10 mg / kg- 0. 20 mg / kg. Conclusion The method is simple,rapid,and accurate,and suitable for the determination in fruits.
出处
《中国卫生检验杂志》
CAS
2015年第14期2277-2279,2283,共4页
Chinese Journal of Health Laboratory Technology
基金
江西省科技计划项目(20121BBG70057)
关键词
超高效液相色谱法
4-氯苯氧乙酸
氯吡脲
2
4-二氯苯氧乙酸
水果
Ultra performance liquid chromatography
4-chlorophenoxyacetic acid
Forchlorfenuron
2
4-dichlorophenoxy-acetic acid
Fruits