期刊文献+

HIV抗体筛查试验阳性样品的复检与确证结果分析 被引量:15

Analysis of the recheck and confirmation results of positive samples in the HIV antibody screening
原文传递
导出
摘要 目的收集某HIV确证实验室2009年-2013年,不同类型艾滋病检测筛查机构送检的HIV抗体呈阳性反应样品,分析其复检及确证结果,探讨目前HIV抗体检测工作的现状及可能存在的问题。方法采用2种ELISA试剂对筛查实验室上送的阳性样品进行复检,其中任何一种试剂检测结果呈阳性反应即以免疫印迹法进行确证检测。结果3 130份初筛阳性样品经复检有2 616份呈阳性反应,复检阳性率为83.6%,各年份复检阳性率差异无统计学意义(χ2=6.35,P=0.174)。选取其中1 860份样品进行确证实验,结果 2009年-2013年各年HIV确证阳性率分别为91.3%、89.9%、92.7%、84.9%、88.1%,各年度确证阳性率差异有统计学意义(χ2=18.01,P=0.003)。结论各年复检阳性率处于一个平稳的水平,各年份确证阳性率不全相同;2种试剂联合检测可以提高确证阳性符合率,降低漏检的风险。 Objective To collect the positive HIV antigen samples from the HIV confirmation laboratory,which were sent for test from different types of AIDS screening institutions from 2009 to 2013,analyze the re- inspection and the confirmation results,investigate the current situation and existing problems in detection of HIV cases. Methods The positive samples were retested with two kinds of ELISA reagents for rechecking. Once positive reaction was obtained with one of the reagents,the samples would be confirmed with western blotting. Results 2 616 cases were confirmed positive in 3 130 re- inspection cases with the re- inspection rate of 83. 8%. The differences of re- inspection rate during the years was not statistically significant( χ2=6. 35,P = 0. 174). 1 860 samples were selected for confirmation,and the result showed that the positive confirmation rate of HIV during 2009- 2013 were respectively 91. 3%,89. 9%,92. 7%,84. 9% and 88. 1%. The differences of confirmation rates during the years were statistically significant( χ2= 18. 01,P = 0. 003). Conclusion The re- inspection positive rates kept a stable level. But all the positive coincidence rates were not the same every year. The joint detection of two reagents can improve the confirming positive cases and reduce the risk of leak test.
出处 《中国卫生检验杂志》 CAS 2015年第14期2408-2410,共3页 Chinese Journal of Health Laboratory Technology
关键词 HIV抗体 筛查实验室 确证符合率 HIV antibody Screening laboratory Confirmation coincidence rate
  • 相关文献

参考文献11

二级参考文献57

共引文献144

同被引文献104

引证文献15

二级引证文献102

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部