摘要
目的建立快速、简便的超高效液相色谱-质谱联用法（UPLC-MS）测定高良姜中高良姜素的含量。方法采用ACQUITY UPLC BEH C18（2.1 mm×50 mm,1.7μm）色谱柱,以乙腈-0.1%甲酸水溶液流动相进行梯度洗脱;柱温30℃;流速0.3 ml/min;采用质谱一级全扫描方式、正离子模式检测。结果高良姜素在0.04～0.15 mg/ml之间与峰面积呈良好的线性关系,相应的回归方程为：y=1.927×105x＋2.832×104（r=0.999 9）;平均回收率为97.85,RSD为2.43。结论该方法快速简便,准确可靠,灵敏度高,可应用于高良姜中高良姜素含量的快速检测及高良姜的质量控制。
Objective To establish a rapid and sensitive ultra performance liquid chromatography-mass spectrometry（ UPLC-MS） method for the determination of galangin in Alpinia officinarum Hance. Methods ACQUITY UPLC BEH C18（ 2. 1 mm × 50 mm,1. 7 μm） column was used with acetonitrile-0. 1% as the formic acid mobile phase in gradient elution. The column temperature was 30 ℃,and the flow rate was 0. 3 ml·min- 1.Full scan MS and positive ion mode detection were used. Results Galangin showed a good linear relationship in the range of 0. 04 ～ 0. 15 mg·ml- 1. The linear regression equation of galangin was y = 1. 927 × 105 x ＋ 2. 832 ×104（ r = 0. 999 9）. The average recoveries was 97. 85; RSD was 2. 43. Conclusions The method is simple,rapid,sensitive,accurate and reliable,which can be applied in the rapid determination of active ingredients and quality control in Alpinia officinarum Hance.
出处
《齐齐哈尔医学院学报》
2015年第22期3364-3365,共2页
Journal of Qiqihar Medical University
基金
黑龙江省自然科学基金项目(B201312)
