摘要
目的:以响应曲面法优化沙棘多糖的提取工艺。方法:在单因素试验的基础上,采用响应曲面法研究提取温度、提取时间和水料比对沙棘多糖提取率的影响。结果:沙棘多糖提取的的最佳工艺条件为:提取温度97.2℃、提取时间60.3 min、水料比15.65∶1,此条件下,沙棘多糖的提取率为10.28%。结论 :响应曲面法工艺简单,得率较高,可用于沙棘多糖的提取。
Objective：To optimize the extraction process of Hippophae fhamnoides L. polysaccharide by re-sponse surface methodology. Methods：Based on the single-factor experiment,the influence of extraction ten-perature,extraction time and ratio of water to material on extraction yield of Hippophae fhamnoides L. polysaccharide was study by response surface methodology. Results：The optimum extraction conditions of Hippophae fhamnoides L. polysaccharide were as follows： the extraction temperature was 97.2℃,the extrac-tion time was 60.3 minutes and the ratio of water to material was 15.65∶1（mL/g）. Under this condition,the yield of polysaccharide from Hippophae fhamnoides L. Was 10.28%. Conclusion：The response surface methodology is simple and with high yield,so it can be used to extract polysaccharide from Hippophae fhamnoides L.
出处
《山东中医杂志》
2015年第8期610-613,共4页
Shandong Journal of Traditional Chinese Medicine
关键词
沙棘
多糖
提取
响应曲面法
polysaccharide
extraction
response surface methodology
