摘要
目的探讨齐墩果酸对胰岛素抵抗人肝癌细胞(Hep G2)关键酶活性的影响。方法将Hep G2细胞分别设正常对照组、模型对照组、二甲双胍组、齐墩果酸组。采用肝糖原测定试剂盒检测齐墩果酸对胰岛素抵抗Hep G2细胞糖原含量的影响;采用葡萄糖-6-磷酸脱氢酶耦联比色法、乳酸脱氢酶耦联比色法及钼酸铵定磷法测定葡萄糖激酶(GK)、磷酸烯醇式丙酮酸羧激酶(PEPCK)和葡萄糖-6-磷酸酶(G-6-Pase)的活性。结果齐墩果酸能够促进胰岛素抵抗Hep G2细胞的葡萄糖消耗,使G-6-Pase及PEPCK活性分别降低54.8%,18.8%,使GK活性和糖原含量分别升高100.6%,98.6%。结论齐墩果酸可降低胰岛素抵抗Hep G2细胞G-6-Pase和PEPCK的活性,抑制糖异生,从而减少细胞内源性葡萄糖的产生。齐墩果酸可提高GK活性,加快糖酵解,增加糖原含量,减轻Hep G2细胞的胰岛素抵抗状态。
Objective To explore the effect of oleanic acid on key enzyme activity in insulin-resistant Hep G2 cells.Methods The Hep G2 cells were divided into normal control,model control,metformin,and oleanic acid groups.Glycogen content in insulin-resistant Hep G2 cell model were detected by hepatic glycogen test kit upon treatment with oleanic acid. Activities of glucokinase( GK),phosphoenolpyruvate carboxylase kinase( PEPCK),and glucose-6-phosphatase( G-6-Pase) were assayed by the glucose 6-phosphate dehydrogenase coupling colorimetric,lactate dehydrogenase coupling colorimetric and ammonium molybdate constant phosphorus methods. Results The oleanic acid enhanced glucose consumption,lowered the activity of G-6-Pase and PEPCK by 54. 8% and 18. 8%,respectively,and increased the activity of GK and glycogen content in also insulinresistant Hep G2 cells by 100.6% and 98.6%,respectively. Conclusion The oleanic acid play a role in lowering PEPCK and G-6-Pase activities and inhibiting glucogenesis,resulting in the reduction of endogenous glucose in the cell. In addition,it can augment the activity of GK,accelerate the process of glucolysis,increase the glycogen content,and alleviate insulin resistance of Hep G2.
出处
《医药导报》
CAS
2015年第9期1139-1141,共3页
Herald of Medicine
