DAPT对Notch2/hes1信号通路在庆大霉素所致大鼠肾损伤及修复过程中表达的影响被引量：4

Effect of DAPT on expression of the Notch2 / hes1 signaling pathways in gentamicin- induced renal injury and repair proess

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摘要目的动态观察DAPT对Notch2/hes1信号通路在庆大霉素诱导肾损伤及修复过程中表达的影响。方法72只成熟大鼠随机分成空白组(生理盐水,3 ml·kg-1·d-1,腹腔注射,持继10 d)、DAPT组(DAPT,5 mg·kg-1·d-1,连用5 d、停用2 d后再用3 d;庆大霉素,120 mg·kg-1·d-1,腹腔注射,持继10 d)和庆大霉素组(庆大霉素,120 mg·kg-1·d-1,腹腔注射,持继10 d)。应用光镜、电镜观察肾小管损伤情况。应用免疫印迹蛋白、实时定量PCR观察大鼠Notch2/hes1信号途径在造模后1 d、14 d、28 d肾脏的动态表达情况。结果肾脏病理切片示:在第1、14 d,与空白组相比,模型组、DAPT组造成的肾脏损伤程度有所不同;在第1、14 d,与模型组相比,DAPT组造成的肾脏损伤程度明显减轻;在28 d,模型组和DAPT组肾小管基本恢复正常。免疫印迹蛋白、RT-PCR示:在第1、14 d,与空白组相比,模型组和DAPT组Notch2/hes1的表达明显增高(P<0.05);在第1、14 d,与模型组相比,DAPT组Notch2/hes1的表达明显减少(P<0.05);在第28 d,各组表达无明显差异(P>0.05)。结论 Notch2/hes1信号通路可能是庆大霉素诱导肾损伤及修复过程中的重要角色之一,DAPT可能通过抑制Notch2/hes1信号通路减轻肾脏损伤并促进肾小管上皮细胞修复。 Objective To dynamically observe effect of DAPT on expression of Notch2/hes1 in gentamicin-induced renal injury and re-pair process. Methods Seventy-two mature rats were randomly divided into the normal group( saline,intraperitoneal injection,once daily, held following the ten days),the DAPT group(DAPT 5 mg/(kg·d),intraperitoneal injection,held following the five days,continuous three days after stop two days. Gen 120 mg/kg,once daily,intraperitoneal injection,continuous ten days)and gentamicin group( Gen 120 mg/kg, once daily,intraperitoneal injection,continuous ten days). The renal damage was observed by light microscopy and electron microscopy. By west-ern-blotting,real-time quantitative PCR,observed expression of the Notch2/hes1 at 1,14,28 day post-modeling. Results Renal biopsy showed:at 1,14 day,the degree of kidney damage is different in the DAPT group and model group compared with the normal group;at 1,14 day,the degree of kidney damage is obvious mitigation in the DAPT group compared with the model group;at 28 day,tubular back to normal in DAPT and gentamicin groups. Western blot protein,RT-PCR shows:at 1,14 day,the level of Notch2/hes1 increased significantly in model group and DAPT group( P <0. 05)compared with the normal group. At 1,14 day,the level of Notch2/hes1 significantly in DAPT group( P <0. 05)compared with the model group. At 28 day,there is no significant difference( P >0. 05). Conclusion Notch2/hes1 signaling pathways may be one of the important role in the gentamicin-induced renal injury and repair process. DAPT may be go through inhibit Notch2/hes1 signa-ling pathways to reduce renal injury and promote tubular epithelial cell repair.

作者黄飞王小琴

机构地区恩施州中心医院肾内科湖北省中医院肾内科

出处《临床和实验医学杂志》 2015年第17期1401-1405,共5页 Journal of Clinical and Experimental Medicine

基金国家临床重点专科建设项目资助

关键词大鼠庆大霉素肾损伤 DAPT Notch2/hes Rats Gentamicin Renal injury DAPT Notch2 /hes1

分类号 R692 [医药卫生—泌尿科学]

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