摘要
赭曲霉毒素A(ochratoxin,OTA)是一类主要由曲霉属和青霉属等真菌产生的次级代谢产物,广泛存在于各种食品及中药材中。其痕量、剧毒、受污染、基质复杂等特点,给实际检测造成了很大困难。在众多检测技术中,胶体金色谱技术以其灵敏度高、特异性好、经济实用、使用方便等优点在OTA快速检测中广泛应用。近年来,随着适配子技术的进步及其在色谱技术中的应用,发展了新型胶体金适配子色谱技术。该文阐述了2种胶体金色谱技术的构造及原理,重点介绍了新型胶体金适配子色谱技术,归纳并比较了它们在OTA快速检测中的应用,展望了胶体金色谱技术的发展趋势,以期为相关研究提供参考。
Ochratoxin A（ OTA） is a toxic secondary metabolite mainly produced by Aspergillus and Penicillium species,existing in a variety of foodstuffs and Chinese medicines. OTA is difficult to be detected in practice because of the characteristics such as trace amounts,toxicity,existing in complex matrices. In the numerous detection technologies,colloidal gold chromatographic techniques are highly sensitive,specific,cost-effective and user-friendly,and are being used increasingly for OTA screening. Recently,with the development of aptamer technology and its application in chromatographic technique,a newly colloidal gold aptamer chromatographic technique has been developed.This review elaborates the structures and principles of both traditional and newly colloidal gold chromatographic techniques,focuses on newly colloidal gold aptamer chromatographic technique,summarizes and compares their use in rapid detection of OTA. Finally,in order to provide a reference for better research of related work,the development trends of this novel technique are prospected.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第15期2945-2951,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金面上项目(81274072
81473346)
2014中医药行业科研专项(201407003)
关键词
OTA
胶体金
适配子
色谱技术
研究进展
ochratoxin A
colloidal gold
aptamer
chromatographic technique
the advance
