摘要
为了探讨壮腰健肾丸的缩尿作用及机制,该实验采用皮下注射150 mg·kg-1剂量的D-半乳糖诱导亚急性衰老模型,并将其分为模型组、缩泉丸组(1.17 g·kg-1·d-1)、壮腰健肾丸高、中、低剂量组(2.39,1.20,0.60 g·kg-1·d-1),另取正常动物设为空白组,连续给药8周。选用代谢笼法测量大鼠24 h尿量及水负荷5 h尿量;采用全自动生化仪检测尿中Na+,Cl-,K+浓度;应用ELISA法测定血清中醛固酮(aldosterone,ALD)、抗利尿激素(antidiuretic hormone,ADH)的含量;采用RT-PCR检测大鼠膀胱组织中P2X1与P2X3mRNA表达量的变化情况。结果显示壮腰健肾丸高、中剂量组对肾虚多尿大鼠24 h尿量及水负荷后5 h内尿量均显著下调(P<0.05,P<0.01);对尿中Na+和Cl-浓度均显著降低(P<0.01);血浆中ALD,ADH含量显著性升高(P<0.05,P<0.01);膀胱组织中P2X1与P2X3mRNA表达量显著下调(P<0.01);壮腰健肾丸低剂量组尿中Na+和Cl-浓度均显著降低(P<0.01)。结果说明壮腰健肾丸可能是通过下调肾虚多尿大鼠膀胱组织中P2X1与P2X3mRNA表达量从而发挥缩尿的作用。
To investigate the urination-reducing effect and mechanism of Zhuangyao Jianshen Wan（ ZYJCW）. In this study,SD rats were subcutaneously injected with 150 mg·kg-1dose of D-galactose to prepare the sub-acute aging model and randomly divided into the model group,the Suoquan Wan group（ 1. 17 g·kg-1·d-1）,and ZYJCW high,medium and low dose groups（ 2. 39,1. 20,0. 60 g·kg-1·d-1）,with normal rats in the blank group. They were continuously administered with drugs for eight weeks. The metabolic cage method was adopted to measure the 24 h urine volume and 5 h water load urine volume in rats. The automatic biochemistry analyzer was adopted to detect urine concentrations of Na＋,Cl-,K＋. The ELISA method was used to determine serum aldosterone（ ALD） and antidiuretic hormone（ ADH）. The changes in P2X1 and P2X3mRNA expressions in bladder tissues of rats were detected by RT-PCR. According to the results,both ZYJCW high and medium dose groups showed significant down-regulations in 24 h urine volume and 5 h water load urine volume in（ P〈0. 05,P〈0. 01）,declines in Na＋and Cl-concentrations in urine（ P〈0. 01）,notable rises in plasma ALD and ADH contents（ P〈0. 05,P〈0. 01） and remarkable down-regulations in the P2X1 and P2X3mRNA expressions in bladder tissues（ P〈0. 01）. The ZYJCW low dose group revealed obvious reductions in Na＋and Cl-concentrations in urine（ P〈0. 01）. The results indicated that ZYJCW may show the urination-reducing effect by down-regulating the P2X1 and P2X3 mRNA expressions in bladder tissues of rats with diuresis caused by kidney deficiency.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第15期3059-3062,共4页
China Journal of Chinese Materia Medica
基金
中央财政支持地方高校发展专项资金项目(财教[2013]338号)
关键词
壮腰健肾丸
肾虚多尿
P2X1
P2X3
Zhuangyao Jianshen Wan
diuresis caused by kidney deficiency
P2X1
P2X3
