摘要
目的：探讨青藤碱联合氟尿嘧啶（5-Fu）对人胃癌SGC7901细胞增殖及Bcl-2、Bax和环氧化酶（COX）-2表达的影响。方法：体外培养人胃腺癌SGC7901细胞系,四甲基偶氮唑蓝法测定对照组、5-Fu组（5-Fu 50 mg/L）、青藤碱低浓度组（青藤碱1.25 mmol/L）、青藤碱中浓度组（青藤碱2.5 mmol/L）、青藤碱高浓度组（青藤碱5 mmol/L）及联合组（5-Fu 25 mg/L＋青藤碱2.5 mmol/L）对SGC7901细胞增殖作用的影响;碘化丙啶染色流式细胞术检测细胞周期变化及凋亡率;免疫细胞化学法测定Bcl-2、Bax和COX-2蛋白表达情况。结果：青藤碱、5-Fu作用SGC7901细胞24、48和72 h后,青藤碱对人胃腺癌SGC7901细胞的增殖具有抑制作用,呈时间、浓度依赖性,与5-Fu联用后协同抑制细胞增殖（P〈0.05-P〈0.01）;5-Fu组、青藤碱各浓度组及联合组的细胞凋亡率均较对照组明显增高（P〈0.01）,其中联合组均较单药组的凋亡率明显降低（P〈0.01）。对照组Bcl-2和COX-2蛋白表达较明显,而青藤碱组、5-Fu组及其联合组染色减弱;与对照组差异均有统计学意义（P〈0.05-P〈0.01）,其中联合组均较单药组阳性率明显降低（P〈0.01）。对照组Bax蛋白表达较弱,青藤碱组、5-Fu组及其联合组阳性率相对增加;与对照组比较差异均有统计学意义（P〈0.01）,且联合组均较单药组阳性率明显升高（P〈0.01）。结论：青藤碱联合5-Fu对人胃腺癌SGC7901细胞增殖具有协同抑制作用,能够上调Bax表达,下调Bcl-2、COX-2表达,可能发挥增敏作用。
Objective： To explore the effects of sinomenine combined with 5-fluomuracil（ 5-Fu） on the proliferation of human gastric gland carcinoma cell line SGC 7901 and expressions of Bcl-2,Bax and COX-2. Methods： The human gastric gland carcinoma cell line SGC 7901 was cultured in vitro,which was divided into the control group,5-Fu group（ 50 mg / L）,low concentration sinomenine group（ 1. 25 mmol/L）,medium concentration sinomenine group（ 2. 5 mmol/L）,high concentration sinomenine group（ 5 mmol/L） and combination group（ 25 mg / L of 5-Fu and 2. 5 mmol / L of sinomenine）. The proliferation of SGC7901 cells was detected by MTT,the cell cycle and apoptosis rate were determined by flow cytometry,and the expressions of Bcl-2,Bax and COX-2 were detected by immunohistochemistry. Results： Sinomenine could inhibit the proliferation of SGC7901 cells after 24 h,48 h and 72 h of treatment,which was dose- and time-dependent,and the treatment with sinomenine combined with 5-Fu could strengthen the inhibitory effects（ P〈0. 05 to P〈0. 01）. Compared with the control group,the apoptosis rates in 5-Fu group,sinomenine groups and combination group increased obviously（ P〈0. 01）,and the apoptosis rate in combination group was significantly lower than that in single drug group（ P〈0. 05 to P〈0. 01）. The positive expressions of Bcl-2 and COX-2 in the control group,and the sinomenine group,5-Fu group and combination group were strong and weak,respectively,the differences of whose were statistical significance（ P〈0. 05 to P〈0. 01）. The positive rates of Bcl-2 and COX-2 expressions in combination group were lower than those in single drug group（ P〈0. 01）. The positive expression of Bax in the control group,and the sinomenine group,5-Fu group and combination group were weak and strong,respectively,the differences of whose were statistically significant（ P〈0. 01）. The positive rate of Bax expression in combination group was higher than that in single drug group（ P〈0. 01）. Conclusions： Sinomenine combined with 5-Fu can enhance the inhibitory effect on SGC7901 cells proliferation,which can further upregulate the expression of Bax,and downregulate the expressions of Bcl-2 and COX-2 protein.
出处
《蚌埠医学院学报》
CAS
2015年第8期998-1001,共4页
Journal of Bengbu Medical College
关键词
胃肿瘤
青藤碱
氟尿嘧啶
细胞增殖
凋亡
stomach neoplasms
sinomenine
5-fluomuracil
cell proliferation
apoptosis
