摘要
山梨醇转运蛋白(SOT)是植物山梨醇运输的关键载体。参考已公布的白梨基因组数据,应用RNA-seq技术在砂梨果肉中鉴别出22个有表达的山梨醇转运蛋白(SOT)基因家族成员,其中10个高表达成员的表达量之和占总表达量的92%。q RT-PCR分析表明上述10个成员的表达存在明显的组织特异性,所有成员在种子中的表达丰度最低;Ppy SOT8在所有组织和器官中都有不同程度表达;Ppy SOT26仅在幼叶中有一定表达。在果实发育期间Ppy SOT2、Ppy SOT8、Ppy SOT10/28和Ppy SOT33的相对表达丰度与果实山梨醇积累呈现良好相关性。4℃贮藏期间果实山梨醇含量趋于下降,与Ppy SOT3、Ppy SOT4、Ppy SOT8、Ppy SOT25、Ppy SOT32和Ppy SOT33表达上调相关。
Sorbitol transporter（SOT）is an essential carrier for sorbitol transport in plants. A total of 22 expressed Ppy SOT members were identified from sand pear（Pyrus pyrifolia Nakai.）flesh by a RNA-seq analysis using Chinese white pear（P. bretschneideri Rehd.）genome as reference,with the top 10 members accounting for 92% of total transcript abundance. Tissue-specific expression patterns of these 10 members were observed via q RT-PCR. All members barely expressed in seed,Ppy SOT8 expressed in all tissues analysed,while the expression of Ppy SOT26 was mostly restricted in young leaves. The transcript abundance of Ppy SOT2,Ppy SOT8,Ppy SOT10/28 and Ppy SOT33 was in a close positive correlation with sorbitol accumulation during fruit development. The content of sorbitol in flesh decreased during cold storage at 4 ℃,and it was related to the increased expression of Ppy SOT3,Ppy SOT4,Ppy SOT8,Ppy SOT25,Ppy SOT32 and Ppy SOT33.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第8期1457-1466,共10页
Acta Horticulturae Sinica
基金
浙江省自然科学基金项目(LY12C15007)
国家现代农业产业技术体系建设专项资金项目(CARS-09-04)
浙江省果品农业新品种选育重大科技专项(2012C12904-2)
关键词
砂梨
山梨醇转运蛋白(SOT)
基因家族
表达特性
果实
糖
Pyrus pyrifolia
sorbitol transporter（SOT）
gene family
expression characteristic
fruit
sugar
