摘要
目的探讨实时荧光RT—PCR法与酶联免疫吸附实验（ELISA—IgM）法在发热伴血小板减少综合征检测中的应用价值。方法连续采集我院2014年78例疑似发热伴血小板减少综合征患者血清138份并对其中49例确诊病例不同病程的91份血清标本，分别应用实时荧光RT-PCR方法和ELISA—IgM方法进行检测，结果进行统计学分析。结果138份标本，实时荧光RT—PCR方法检测阳性率为51．45％，ELISA—IgM方法检测阳性率为43．48％，两种方法的检测有统计学意义（P〈0．05）。在49例确诊病例中两种方法检测阳性率均受病程影响，病程早期（8d内）实时荧光RT—PCR方法检出阳性率高于ELISA—IgM法，分组检验具有统计学意义；但随着病程的延长ELISA—IgM法的检测阳性率有升高的趋势。结论在发热伴血小板减少综合征病例的早期试验诊断中应首先使用实时荧光RT-PCR法，而ELISA—IgM法是其必要的补充，随着病程的延长后者将成为重要的诊断手段。
Objective To compare the difference of the positive rates between real-time RT-PCR and ELISA-IgM methods in diagnosis of severe fever with thromboeytopenia syndrome （SFTS） cases. Method 138 serum samples from 78 suspected acute SFTS patients were collected continuously in our hospital, 2014. And 91 serum samples from 49 confirmed cases were detected by real-time RT-PCR and ELISA-IgM, simultaneously. The results were analyzed by Software SPSS17.0. Result 138 serum samples were detected by the real-time RT-PCR and ELISA-IgM methods, and the positive rate was 51.45%, 43.48% respectively. There was significant difference between their positive rates （P 〈 0. 05）. The courses of disease influenced the positive rates notablely in 49 confirmed cases between the real-time RT-PCR and ELISA-IgM assays. When the serum samples were collected within 8 days onset of illness to be detected, the positive rate of real-time RT-PCR method was higher than that of ELISA-IgM method. While the positive rate of ELISA-IgM showed a rising tendency along with the course extension. Conclusion Real-time RT-PCR would be the first choice for the early diagnosis of suspected SFTS cases. While ELISA-IgM methods could be the necessary supplemental assay and it would be the important diagnostic method as the extension of the course of SFTS.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2015年第4期351-353,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
血小板无力症
逆转录聚合酶链反应
酶联免疫吸附测定
Thromboasthenia
Reverse transcriptase polymerase chain reaction
Enzyme-linkedimmunosorbeat assay
