摘要
目的建立RP-HPLC法测定保肝丸中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1和野黄芩苷的量,对保肝丸的制剂质量提供保障。方法采用安捷伦Zorbax SB-C18（150 mm×4.6 mm,5μm）色谱柱;流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,体积流量1.0 m L/min;紫外检测波长为203 nm;柱温30℃;进样量为5μL。结果三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1和野黄芩苷的最低检测质量浓度分别为0.287、0.126、0.182、0.305 mg/L,线性范围分别为4.427-141.668、6.055-193.750、3.255-104.167、5.729-183.333 mg/L。供试样品中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1和野黄芩苷的平均回收率分别为101.76%、99.66%、99.43%、101.26%;精密度RSD分别为1.81%、1.79%、1.39%、1.51%;重复性试验RSD分别为1.71%、1.86%、0.97%、1.63%;稳定性试验RSD分别为1.62%、1.25%、1.10%、1.41%。供试样品每丸含三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1和野黄芩苷的平均量分别为4.303、31.729、20.776、1.071μg。结论该方法简便、灵敏度高、重复性好、平均回收率高,是检测三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1和野黄芩苷质量浓度的可信方法。
Objective To establish an RP-HPLC method for determining the mass concentration of notoginsenoside R1, ginsenoside Rg1, Rb1, and scutellarin in Baogan Pill, and to provide the quality guarantee for Baogan Pill. Methods Agilent Zorbax SB-C18（150 mm × 46 mm, 5 μm） column were used. Acetonitrile and 0.1% phosphoric acid water-solution were used as mobile phase of gradient elution, the volume flow was at 1.0 m L/min; Ultraviolent determination wavelength was 203 nm; Column temperature was at 30 ℃, and injection volume was 5 μL. Results The lowest detection limit in notoginsenoside R1 was 0.287 mg/L, the linear range was 4.427—141.668 mg/L; The lowest detection limit in ginsenoside Rg1 was 0.182 mg/L, the linear range was 6.055—193.75 mg/L; The lowest detection limit in ginsenoside Rb1 was 0.182 mg/L, the linear range was 3.255—104.167 mg/L; The lowest detection limit in scutellarin was 0.305 mg/L, the linear range was 5.729—183.333 mg/L. The average recovery of notoginsenoside R1, ginsenoside Rg1, Rb1 and scutellarin in Baogan Pill respectively were 101.76%, 99.66%, 99.43%, and 101.26%; The precision RSD values respectively were 1.81%, 1.79%, 1.39%, and 1.51%; The repeatability RSD values respectively were 1.71%, 1.86%, 0.97%, and 1.63%; The stability RSD values respectively were 1.62%, 1.25%, 1.10%, and 1.41%. The average amounts of notoginsenoside R1, ginsenoside Rg1, Rb1, and scutellarin in the selected samples per pill respectively were 4.303, 31.729, 20.776, and 1.071 μg. Conclusion The method is believable for determining the mass concentration of R1, Rg1, Rb1, and scutellarin with its simplicity, sensibility, repeatability, and better recovery rate.
出处
《中草药》
CAS
CSCD
北大核心
2015年第16期2417-2420,共4页
Chinese Traditional and Herbal Drugs
基金
天津市应用基础与前沿技术研究计划(14JCYBJC24800)
