摘要
目的探讨survivin反义寡核苷酸(ASODN)在活体内对人卵巢癌细胞种植性转移能力和腹腔移植瘤形成的影响,分析其可能的作用机制。方法构建人卵巢癌SKOV3细胞株裸鼠腹腔移植瘤模型,实验组给予survivin ASODN腹腔注射,同时对照组给予生理盐水腹腔注射,比较两组动物腹腔移植瘤形成和生长情况的改变,并通过蛋白印迹法检测两组腹腔移植瘤组织中白细胞介素6(IL-6)、信号转导与活化因子3(STAT3)、磷酸化的信号转导与活化因子3(p-STAT3)、survivin蛋白表达的改变。结果Survivin ASODN组人卵巢癌细胞裸鼠腹腔移植瘤数量和重量都较对照组明显减小(P<0.05),IL-6、STAT3、p-STAT3、survivin蛋白表达均明显下降(P<0.05)。结论 Survivin ASODN在活体内能有效降低人卵巢癌细胞侵袭和种植性转移能力,能有效抑制卵巢癌腹腔移植瘤的生长,这些作用是通过抑制IL-6/STAT3信号通路的活性来完成的。靶向survivin基因的反义核苷酸治疗将成为临床防治卵巢癌复发和转移重要手段,有重要的临床价值。
Objective To observe the effect of anti-survivin oligonucleotides （ASODN） on the invasion and growth of peritoneally implanted ovarian cancer cell xenografts in nude mice. Methods Nude mouse models bearing peritoneally implanted ovarian cancer cell （SKOV3） xenografts were established and subjected to intraperitoneal injection of survivin ASODN or saline （control）. The number and weight of the intraperitoneal xenografts were compared between the two groups. The expressions of interieukin （IL-6）, signal transducer and activator of transcription3 （STAT3）, phosphorylated STAT3 （p-STAT3）, and survivin protein in the tumor tissues were detected with Western blotting in both groups. Results Compared with those in the control group, the number and weight of the intraperitoneal xenografts were significantly reduced in ASODN group （P〈0.05）. ASODN treatment also resulted in significantly lowered protein levels of IL-6, STAT3, p-STAT3, and survivin in the tumor tissues （P〈0.05）. Conclusion Survivin ASODN can suppress the invasion and migration capacity of ovarian cancer cells and inhibit peritoneal metastasis of the tumor in nude mice possibly though down-regulation of IL-6/ STAT3 signaling pathway.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2015年第8期1211-1214,F0003,共5页
Journal of Southern Medical University
基金
河北省自然科学基金(H2013206198)
