摘要
为了合理开发利用人工培养发菜,比较分析了人工液体培养发菜和野生发菜的营养成分。通过高效液相色谱(HPLC)、电感耦合等离子体发射光谱(ICP-OES)、苯酚硫酸、凯氏定氮和索氏抽提法测定了2种发菜的Ca、Mg、Cu、Zn、Sr、Fe、Se元素、氨基酸、粗多糖、蛋白质、脂肪的含量。结果表明:人工液体培养发菜中粗蛋白、脂肪含量分别为26.17%、0.23%,而野生发菜中为23.05%、0.31%;人工培养发菜的胞外多糖含量为36.95 mg/L,人工培养发菜与野生发菜的荚膜多糖含量分别为15.03 mg/kg和13.84 mg/kg,氨基酸总量分别为21.99 mg/g和21.20 mg/g,其中必需氨基酸含量分别为11.73 mg/g和7.97 mg/g。人工培养发菜的Mg、Cu、Zn、Se含量优于野生发菜,分别是野生发菜的2.9、3.4、3.8、1.6倍。综合分析认为,人工液体培养发菜能分离较多的胞外多糖;粗蛋白、脂肪、荚膜多糖、氨基酸和Ca、Sr、Fe元素含量与野生发菜相近。
This study aimed to compare and analyze the nutrient contents of cultured and wild Nostoc flagelliforme. Crude protein,fat,polysaccharide were measured in the cultured and wild Nostoc flagelliforme were analyzed.The contents of amino acids,Ca,Mg,Cu,Zn Sr,Fe and Se were determined using HPLC and ICP-OES. The results indicated that crude protein and fat contents accounted for 26. 17% and 0. 23%（ w / w） in cultured Nostoc flagelliforme,while 23. 05% and 0. 31% in the wild Nostoc flagelliforme. Exopolysaccharide contents reached 36. 95 mg / L under cultivated conditions. Capsular polysaccharide contents in cultured and wild Nostoc flagelliforme were 15. 03 mg / kg and 13. 84 mg / kg,respectively. The total amino acids contents were 21. 99 mg / g and 21. 20 mg / g,essential amino acids contents were 11. 73 mg / g and 7. 97 mg / g. The elements of Ca,Mg,Cu,Zn,Sr,Fe,Se in the cultured Nostoc flagelliforme were rich,and Mg,Cu,Zn,Se were 2. 9,3. 4,3. 8,and 1. 6 times higher than that of in wild one. In conclusion,the cultured Nostoc flagelliforme can secrete more exopolysaccharide. The contents of crude protein,fat,protein,amino acid,Ca,Sr,Fe were similar with wild Nostoc flagelliforme. Cultured Nostoc flagelliforme has great prospects for development.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第8期181-185,共5页
Food and Fermentation Industries
基金
河南省重点科技攻关计划项目(No.142102110038)
河南科技大学博士科研启动基金
河南科技大学大学生研究训练(SRTP)项目(No.2014117)
关键词
发菜
营养成分
分析
Nostoc flagelliforme
nutritive composition
analysis
