摘要
目的 ：分析ffh基因沉默对变形链球菌耐氟菌株体外耐酸能力的影响。方法 ：利用电穿孔法,将si RNA转入UA159-FR,使其与ffh基因靶向位点结合,筛选出含ffh基因沉默的变形链球菌耐氟菌株,与UA159-FR的标准菌液分别在不同p H值（以0.5为间隔,p H为3.5~7.5）的BHI液体培养基中37℃微需氧（95%N2、5%CO2）培养24 h,离心,采用p H计测定培养物上清的终末p H值;5 m L生理盐水稀释细菌沉淀,用紫外分光光度计测定600 nm处的吸光度,采用SPSS 17.0软件包对所得数据进行统计学分析。结果：原菌株UA159-FR与ffh基因沉默的变形链球菌UA159-FR相比,Δp H差异显著（P〈0.05）,且前者高于后者。两者比较p H=3.5~5.0时,OD600有显著差异（P〈0.01）;p H=5.5~7.5时,OD600有显著差异（P〈0.05）,且两者生长趋势相似。结论：ffh基因沉默对变形链球菌耐氟菌株的耐酸性有一定影响。
PURPOSE： To analyze the effect of ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans in vitro. METHODS： By using electroporation, UA159-FR was transformed and combined with targeted site of ffh gene sequence, and the best piece of si RNA for fluoride resistant Streptococcus mutans was screened. In different values of p H of BHI, they were cultured for 24 hours with UA159-FR respectively, and then centrifugated to determine the p H and OD600.SPSS17.0 software package was used to analyze the data. RESULTS： The aciduricity of UA159-FR had significant differences compared with ffh gene silencing for UA159-FR in Δp H（P〈0.05）, and the former was higher than the latter. At p H=3.5 ~5.0, P〈0.01; at p H=5.5 ~7.5, P〈0.05. Significant differences were noted in OD600 and their growth tendency were similar. CONCLUSIONS： The aciduricity of fluoride resistant Streptococcus mutans has significant effect when the ffh gene is silenced.
出处
《上海口腔医学》
CAS
CSCD
2015年第4期395-399,共5页
Shanghai Journal of Stomatology
基金
国家自然科学基金(3A411X463431)~~
关键词
FFH
基因沉默
变形链球菌耐氟菌株
耐酸性
ffh
Gene silencing
Fluoride resistant Streptococcus mutans
Aciduricity
