摘要
目的建立高效液相色谱法同时测定归芍地黄丸中莫诺苷、马钱苷、芍药苷和丹皮酚的含量。方法色谱柱为Waters Symmetry C18（250 mm×4.6 mm,5μm）,流动相为乙腈-0.3%磷酸溶液,梯度洗脱,柱温为35℃,流速为0.8 mL·min-1,检测波长为240 nm,外标法计算含量。结果莫诺苷进样量在0.010 48~2.62μg与峰面积线性关系良好（r=0.9999）,平均加样回收率为102.8%,RSD为1.4%;马钱苷进样量在0.00868~2.17μg与峰面积线性关系良好（r=0.9999）,平均加样回收率为103.6%,RSD为1.1%;芍药苷进样量在0.007 696~1.15μg与峰面积线性关系良好（r=0.9943）,平均加样回收率为102.3%,RSD为2.1%;丹皮酚进样量在0.018~2.25μg与峰面积线性关系良好（r=0.9998）,平均加样回收率为102.2%,RSD为1.6%。结论该方法准确、简便、可行、重复性好,可有效控制归芍地黄丸的质量。
Objective To develop an HPLC method to simultaneously determine the content of morroniside, loganin, paeoniflorin and paeonol in Guishao Dihuang pills. Methods The column was Waters Symmetry C18 （250 mm × 4.6 mm, 5 lam）, and the mobile phase was acetonitrile and 0.3% phosphoric acid with gradient elution. The column temperature was 35 ℃, the flow rate was 0.8 mL · min - 1, the detection wavelength was 240 nm, and the external standard method was used. Results Good linearity was shown at 0.010 48 - 2.62μg （r = 0.9999） for morroni- side, 0.008 68 - 2.17μg （r = 0.9999） for loganin, 0.007 696 - 1.15μg （r = 0.9943） for paeoniflorin and 0.018 - 2.25 μg （r =0.9998） for paeonol. The average recoveries were 102.8% （RSD = 1.4%）, 103.6% （RSD =- 1.1%）, 102.3% （RSD = 2.1%） and 102.2% （RSD = 1.6%）, respectively. Conclusion This method is accurate, simple, and feasible with good repeatability. It can be used in the quality control of Guishao Dihuang pills.
出处
《中南药学》
CAS
2015年第8期855-858,共4页
Central South Pharmacy
基金
国家药典委员会中国药典2015年版药典科研课题(No.1181)
