摘要
The effect of matrix attachment regions (MARs) on foreign gene expression in transgenic plants was studied, The beta-glucuronidase (GUS) gene (uidA) was flanked by the MARs isolated from the genome of maize to form plant expression vector. The vectors with and without MARs were transferred into tobacco ( Nicotiana tabacum L.) through Agrobacterium-mediated transformation procedure. GUS activity assays indicated that MARs could increase expression level of uidA gene. The mean GUS activity could be increased twofold as compared to that of transformants without MARs, and the highest GUS activity of transformant could reach tenfold. The correspondence between GUS activity and mRNA accumulation was positive and indicated that MARs could improve transcription of foreign gene.
为研究核基质结合区 (matrixattachmentregion ,MAR)在转基因植物中的功能 ,将来自玉米基因组的MAR序列构建在植物表达载体T_DNA中 ,并将报告基因β_葡糖醛酸酶 (β_glucuronidase ,GUS)基因 (uidA)插入两段MARs序列之间。将此载体与不包含MARs序列的植物表达载体分别转化烟草 (NicotianatabacumL .)。GUS活性检测表明 ,MARs可以显著提高外源基因uidA在转基因烟草中的表达水平 ,平均表达水平提高 2倍 ,最高单株活性可达 10倍。并且转基因植株GUS活性高低与稳定mRNA的量成正比 ,表明MARs在转录水平提高基因表达。
基金
"8 6 3"项目 ( 2 0 0 1AA2 12 0 41)
国家自然科学基金重点项目 ( 399890 0 1)
国家转基因植物研究与产业化专项基金 (J99 B 0 0 4)。~~
