摘要
目的探讨丙戊酸钠对人视网膜细胞瘤Y79细胞凋亡及其机制研究。方法用噻唑蓝（MTT）法检测不同浓度的VPA（浓度分别为0、0．5、1．0、2．0、4．0、8．0mmol／L）对人视网膜细胞瘤Y79细胞增殖影响；流式细胞仪（FCM）对人视网膜细胞瘤Y79细胞凋亡水平的影响；WesternBlot检测Y79细胞中P53、Bax和Bcl-2的表达水平。结果MTT结果显示不同浓度VPA对人视网膜细胞瘤Y79细胞均具有抑制其增殖作用，其中2．0mmol／LVPA处理Y79细胞24h、48h和72h后细胞存活率分别为84．25％、76．41％和59．36％（F=40．18、35．74和42．65，P均〈0．05）。FCM结果显示VPA诱导Y79细胞24h的凋亡水平为26．59％（P〈0．05）。Western Blot结果显示Y79细胞中P53和Bax均显著上升，Bcl-2无显著变化。结论丙戊酸钠能抑制Y79细胞的增殖和诱导其凋亡的作用，其机制与激活P53和Bax有关。
Objective To investigate the mechanism of Valproic Acid （VPA） on retinoblastoma Y79 cell＇ s proliferation and apoptosis. Method MTr was used to detect the proliferation of Y79 cell after treated with different concentration of VPA （0, 0.5, 1.0, 2.0, 4.0 and 8.0 mmol/L）. FCM was used to analyze the apoptosis level of Y79 cell. Western blot was used to detect P53, Bax and Bcl-2 in Y79 cell. Results MTT results showed that VPA could inhibit retinoblastoma Y79 cell＇ s proliferation after treated with different concentration of VPA. The survival fraction of Y79 cell was 84.25 %, 76.41% and 59.36% after 24 h, 48 h and 72 h incubated with VPA, respectively （ P 〈 0. 05 ）. The apoptosis level induced by VPA was 26.59% 24 h later in Y79 cell. P53 and Bax were largely increased （ P 〈 0. 05 ） , while Bcl-2 has no significant change （ P 〉 0. 05）. Conclusion VPA could inhibit retinoblastoma Y79 cell＇ s proliferation and induce apoptosis, which is associated with activation of P53 and Bax.
出处
《国际免疫学杂志》
CAS
2015年第5期427-429,共3页
International Journal of Immunology
