摘要
目的观察桂枝汤及黄连解毒汤对自发性糖尿病大鼠(GK大鼠)心肌基膜厚度、心肌核因子-κB(NF-κB)、Ⅳ型胶原表达的作用,探讨有关炎症损伤的机制。方法以Wistar大鼠10只作为正常对照组,40只GK大鼠随机分为GK对照组、二甲双胍组、黄连解毒汤组、桂枝汤组,分别以相应药物灌胃给药12周,检测各组血糖、NF-κB、Ⅳ型胶原阳性表达程度及基膜厚度。结果 GK大鼠血糖升高,NF-κB阳性表达增强,Ⅳ型胶原蛋白表达增强,基底膜厚度增加。二甲双胍、黄连解毒汤均可降低GK大鼠血糖(P<0.01)。黄连解毒汤和桂枝汤均能降低NF-κB及Ⅳ型胶原蛋白表达、降低基底膜厚度与GK大鼠对照组比较差异有统计学意义(P<0.01,P<0.05)。在抑制NF-κB阳性表达方面,桂枝汤和黄连解毒汤疗效无统计学意义。在抑制Ⅳ型胶原蛋白表达、降低基底膜厚度方面,桂枝汤作用强于黄连解毒汤(P<0.01,P<0.05)。结论桂枝汤和黄连解毒汤均可以抑制NF-κB阳性表达,抑制Ⅳ型胶原蛋白表达,降低基膜厚度,且桂枝汤优于黄连解毒汤,显示了其调和营卫治法的独特作用。
Objective To observe the effect of Guizhi decoction and Huanglianjiedu decoction on the thickness of myocardial basement membrane and the expression of NF - κB and type IV collagen,and to explore the mechanism of inflammatory injury in Spontaneous diabetic rats（GK rats）. Methods Ten Wister rats were included in the control group,while forty GK rats were randomly divided into GK control group,met-formin group,Huanglianjiedu decoction group and Guizhi decoction group. Each group was respectively ad-ministrated the corresponding dose of drugs for 12 weeks. Then blood glucose,the degree of positive expres-sion of myocardial NF - κB and type IV collagen and the changes of basement membrane thickness were de-tected. Results Compared with control group,blood glucose,the positive expression of NF - κB and type IV collagen protein and basement membrane thickness in GK group were increased. Metformin and Huanglian-jiedu decoction decreased blood glucose of rats（P ﹤ 0. 01）. Huanglianjiedu decoction and Guizhi decoction could reduce the positive expression of NF - κB and type Ⅳ collagen,as well as basement membrane thick-ness（P ﹤ 0. 01 or P ﹤ 0. 05）. In the aspect of inhibiting positive expression of NF - κB,there was no differ-ence between Huanglianjiedu decoction and Guizhi decoction. In terms of the inhibiting positive expression of type IV collagen and the reduction of basement membrane thickness,the effect of Guizhi decoction was better than Huanglianjiedu decoction（P ﹤0.01 or P ﹤0.05）. Conclusion Both Huanglianjiedu decoction and Guizhi decoction could inhibit the positive expression of NF - κB and type Ⅳ collagen,and could decrease the base-ment membrane thickness in diabetic rats. But in terms of the inhibiting positive expression of type IV colla-gen and the reduction of basement membrane thickness,the effect of Guizhi decoction is better than that of Huanglianjiedu decoction,which shows the unique role of harmonizing construction and defense therapy.
出处
《世界中西医结合杂志》
2015年第7期999-1002,共4页
World Journal of Integrated Traditional and Western Medicine
基金
山东省优秀中青年科学家基金资助项目(2005BSB02023)
国家自然科学基金资助项目(81072962)
关键词
基膜
Ⅳ型胶原蛋白
核因子-κB(NF-κB)
桂枝汤
黄连解毒汤
Basement membrane
Type IV collagen protein
Nuclear factor - kB（NF - kB）
Guizhi decoction
Huanglianjiedu decoction
