摘要
目的：研究细菌性脑膜炎中蛋白络氨酸磷酸酶（SHP2）的表达意义。方法依据随机数字表法将90只大鼠分为脑膜炎组（72只）和健康对照组（18只）两组，用反转录（RT）-PCR、Western印迹法、免疫组织化法对脑膜炎组和健康对照组大鼠不同时间点脑组织SHP2表达进行检测，然后对SHP2蛋白表达和肿瘤坏死因子-α（TNF-α）、白细胞（WBC）计数的关系进行观察分析。结果脑膜炎组大鼠皮层SHP2 mRNA表达（0．035±0．020）、（0．200±0．049）、（0．129±0．032）、（0．057±0．039）均明显比健康对照组（0．031±0.028）高（F＝12．74，P＜0．05）；第三脑室周围 SHP2阳性染色细胞明显比健康对照组多（χ2＝5．02，P＜0.05）；皮层SHP2蛋白表达与脑脊液TNF-α浓度、WBC计数的相关系数分别为0．08（P＞0．05）和0．77（t＝4．303，P＜0．05）。结论细菌性脑膜炎的病理生理过程有中SHP2参与，主要作用可能是抑制炎症、修复炎性反应，可作为病情变化的参考指标。
Objective To study the significance of protein tyrosine phosphatase （SHP2）expression in bac-terial meningitis.Methods 90 rats were divided into meningitis group （72）and healthy controls （18）two groups based on the random number table,The SHP2 expression in rat brain tissue at different time points of meningitis group and healthy control group were tested by reverse transcription （RT）a PCR,Western blotting,immunohistochemical methods,then the relationship between SHP2 protein expression and tumor necrosis factor-α（TNF-α）,white blood cell （WBC）counts were observed and analyzed.Results The cortical SHP2 mRNA expression of meningitis rat in-cluding （0.035 ±0.020）,（0.200 ±0.049）,（0.129 ±0.032）and （0.057 ±0.039）,were significantly higher than those of the healthy control group （0.031 ±0.028）（F=12.74,P〈0.05）;the third periventricular SHP2 positive cells were significantly more than those of the healthy control group （χ2 =5.02,P〈0.05）;the correlation coefficient of cortical SHP2 protein expression and concentration of CSF TNF -α,the correlation coefficient of cortical SHP2 protein expression and WBC counts were respectively 0.08（P〉0.05）and 0.77 （t=4.303,P〈0.05）.Conclusion SHP2 participates in pathophysiology of bacterial meningitis,the main role may be in suppressing inflammation and repairing inflammatory response,it can be used as a reference indicator of condition changes.
出处
《中国基层医药》
CAS
2015年第17期2646-2648,共3页
Chinese Journal of Primary Medicine and Pharmacy
