摘要
根据GenBank中已发表的猪伪狂犬病病毒（PRV）gE、gI基因的序列设计了2对引物,对PRV NP株的gE、gI基因进行了PCR扩增、回收、克隆、测序,测序结果与预期的PRVgE、gI基因片段相符。同源性比对分析结果显示,PRV NP株gE、gI基因推导的氨基酸序列与国内分离的PRV毒株的同源性分别为95.7%~99.8%、89.9%~99.5%。遗传进化树分析和氨基酸序列比对结果发现PRV NP株的gE氨基酸序列发生变化的位点与2012年国内分离到的PRV流行株相同,从而推测NP株为PRV变异毒株,本研究为PRV的流行病学调查分析奠定了基础,也为开发科学、有效的新型猪伪狂犬病（PR）疫苗提供科学依据。
According to published gE and gI gene sequences of pseudorabies virus（PRV）in GenBank,we designed two pairs of primers for PCR amplification of gEand gIgenes of PRV NP isolate,after PCR products recycling,cloning and sequencing,the sequencing results were consistent with expectations of PRVgEand gI genes.Homology comparison analysis results revealed that compared with the domestic PRV strains,the homologies of gE and gI amino acids of PRV NP isolate were 95.7%to 99.8% and 89.9% to 99.5%,respectively.Phyogenetic tree analysis and amino acid sequence alignment results found that the gE amino acid sequence site changes of PRV NP isolate were the same with the PRV strains which were isolated from domestic in 2012,thus we could speculate that PRV NP isolate had mutanted.This study had laid the foundation for the epidemiological investigation and analysis of PRV,also provided the scientific basis for the development of scientific,effective and new pseudorabies vaccine.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第9期2262-2269,共8页
China Animal Husbandry & Veterinary Medicine
基金
福建省农业科学技术项目(2014-01)
福建省自然科学基金项目(2013J05042)
