摘要
提出了鸡大肠组织在使用双极型电热闭合系统进行闭合过程中的生物阻抗变化情况与最终闭合效果。通过控制双极型闭合系统的能量输出时间,来观察不同作用时间下鸡大肠组织Cole曲线的变化情况,并通过Cole函数中的4个参数来计算其等效生物阻抗模型。同时结合闭合过程中的温度变化情况以及闭合结束后的肠道爆破压来推断组织内部的变化信息,并且验证肠道闭合效果。结果表明在整个闭合过程中鸡大肠组织呈现三个变化阶段:第一阶段细胞外液电阻Re和细胞内液电阻Ri上升,细胞膜电容Cm减小;第二阶段内Re和Ri维持小幅波动,而Cm缓慢上升;到了第三阶段Re和Ri再次急剧上升而Cm则大幅下降。根据等效生物阻抗模型、组织温度以及爆破压变化数据,推断出细胞内部在这三个阶段所发生的变化,且实验证明双极型电热闭合系统能够安全有效地闭合肠道组织。
Base on bipolar electro-thermal sealing system, the bioimpedance and sealing effects of chicken intestines during electro-thermal sealing were analyzed in this paper. The changes of Cole curve of chicken intestines at different stages were observed by controlling the energy output duration of bipolar sealing system. And the equivalent bioimpedance models of each stage were calculated according to the four parameters in the Cole function. Meanwhile, the changes inside the tissues were also obtained and the sealing effects of intestine were verified by comprehensively analyzing the changes of temperature during sealing and the intestine burst pressure after sealing. The results showed that there were three transition phases during the whole sealing process. In the first phase, both extracellular resistance, Rc, and intracellular resistance, Ri, were increased, and the capacitance of cell membrane （Cm） was decreased. In the second phase, Rc and Ri fluctuated in a narrow range while Cm increased slowly. In the final phase, Rc and Ri increased dramatically again and Cm notably declined. Based on the analysis of equivalent bioimpedance models, tissue temperature, burst pressure, the intracellular changes at these three phases were deduced. And the experience proved that bipolar electro-thermal sealing system could seal the intestine effectively and safely.
出处
《中国医学物理学杂志》
CSCD
2015年第5期622-628,共7页
Chinese Journal of Medical Physics
基金
国家自然科学基金(51377024)
上海市自然科学基金(14ZR1428300)
上海市科委生物医药领域科技支撑项目子课题(13441900802)
上海市科委基础研究重点项目子课题(13JC1407202)
关键词
生物阻抗
大肠
双极型电热闭合系统
Cole函数
爆破压
bioimpedance
intestine
bipolar electro-thermal sealing system
Cole function
burst pressure
