摘要
目的:比较寻常型银屑病皮损组织与正常皮肤组织中Nestin、NF-κBp65和STAT3蛋白表达的差异。方法:应用免疫组化技术检测14例寻常型银屑病皮损和14例正常皮肤标本组织中Nestin、NF-κBp65和STAT3蛋白的定位及表达水平。结果:Nestin蛋白定位于表皮基底层个别干细胞的胞质内,NF-κBp65和STAT3蛋白定位于表皮基底细胞和棘层细胞的胞核及胞质。寻常型银屑病皮损组织中Nestin[(167.51±12.93)vs(114.38±10.85)]、NF-κBp65[(127.48±13.87)vs(101.27±10.15)]和STAT3[(196.34±10.70)vs(124.63±19.93)]蛋白的表达水平均高于正常皮肤组织,差异有统计学意义(t=12.977,6.288,13.072;P均<0.001)。寻常型银屑病患者皮损组织和正常皮肤标本组织中NF-κBp65与STAT3蛋白表达均呈正相关(r=0.960和0.829,P均<0.001)。结论:寻常型银屑病皮损组织中Nestin、NF-κBp65和STAT3蛋白表达水平较高,抑制它们的表达可能是治疗该病的有效手段。
Aim: To compare the expression levels of Nestin, NF-κBp65 and STAT3 in psoriatic lesions and normal samples. Methods: Immunohistochemical technique was used to determine the expressions of Nestin, NF-κBp65 and STAT3 in 14 psoriatic lesions and 14 normal samples. Results: The Nestin was located in the cytoplasm of respective stem cells in the basic cell layer, while that of NF-κBp65 or STAT3 was located in nuclei and cytoplasm in some basic layer cells and prickle layer cells. The expression levels of Nestin, NF-κB and STAT3 in psoriatic lesions were significantly higher than that in the normal samples [ Nestin(167. 51 ± 12. 93) vs (114. 38 ± 10. 85),NF-κBp65 (127. 48 ± 13. 87) vs (101. 27 ± 10. 15) and STAT3(196. 34 ± 10. 70) vs (124. 63 ± 19. 93), t = 12. 977,6. 288,and 13. 072,all P 〈 0. 001]. There was positive correlation between NF-κBp65 and STAT3 in psoriatic lesions and normal samples ( r = 0. 960 and 0. 829,P 〈 0. 001). Conclusion: The expression levels of Nestin,NF-κB and STAT3 are significantly higher in psoriatic lesion, and inhibiting their expressions may be valid manipulation for treatment of psoriasis vulgaris.
出处
《郑州大学学报(医学版)》
CAS
北大核心
2015年第4期522-525,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省自然科学基金项目2011A520042