摘要
目的探讨血清中活性维生素B12检测在维生素B12缺乏中的应用价值。方法采用酶免疫发光分析竞争法检测血清中维生素B12和叶酸的含量,及ELISA法检测血清活性维生素B12,通过ROC曲线分析活性维生素B12在诊断维生素B12缺乏中的特异度和敏感度;并且通过回归分析活性维生素B12水平与性别、年龄、总维生素B12及叶酸的相关性;统计分析各疾病组中活性维生素B12含量的累积百分比。结果血清活性维生素B12判断维生素B12缺乏的ROC曲线下面积为0.983,敏感度为60.0%,特异性为99.2%;活性维生素B12与总维生素B12和叶酸存在良好的正相关,P<0.001,与年龄、性别不相关;各病例组中血液病组的活性维生素B12含量累计百分比最高,其次为神经系统疾病组。结论血清活性维生素B12是用于临床维生素B12缺乏诊断较敏感、特异的指标,在血液病、神经系统疾病患者中,其临床应用价值更突出。
Objective To evaluate the value of the serum active vitamin B12 in diagnosing the vitamin B12 deficiency. Methods EIA was used to measure the levels of total vitamin B12 and folate. The active vitamin B12 was measured by ELISA. Evaluate the specifility and the sensitivity of the active vitamin B12 in the diagnosis of the vitamin B12 deficiency with ROC curve.Through Logistic regression,to analyze the correlation between the levels of the active vitamin B12 and the different sex,age the levels of total vitamin B12 and folate. Then the statistic analysis was done on the basis of the cumulative percentage of the concentration of active vitamin B12 in different groups. Results The area under the ROC curve of B12 deficiency was 0. 983,and the sensitivity was 60. 0%,the specificity was 99. 2%; active vitamin B12 levels were positively correlated with the total vitamin B12 and folate（ P〈0. 001）,there were no correlation with age and gender; the highest cumulative percentage of each group was the blood disease,and the nervous system diseases ranked secondly. Conclusion The active vitamin B12 can be used to diagnose the vitamin B12 deficiency as a sensitive and specifided marker,which also showed great value in patients with blood and nervous system disease.
出处
《中国卫生检验杂志》
CAS
2015年第17期2910-2912,共3页
Chinese Journal of Health Laboratory Technology
