摘要
目的:研究制首乌含药血清对L02肝细胞增殖和凋亡的影响。方法:采用皮下注射氢化可的松,建立大鼠肾阴虚模型,分为空白对照组、制首乌低、中、高剂量组(10.8,21.6,43.2 g·kg-1)。连续给药7 d后处死大鼠,制备含药血清。分别采用MTT法测定L02肝细胞线粒体功能、流式细胞术检测肝细胞凋亡率及显微镜观察肝细胞形态结构。结果:15%的肾阴虚高剂量组含药血清作用48 h后对L02肝细胞增殖具有明显抑制作用,抑制率为16.83%(P<0.05)。肾阴虚中、高剂量组的细胞凋亡率显著性升高,分别为19.6%(P<0.05)和27.8%(P<0.01)。结论:制首乌对证治疗肾阴虚证侯,当给药剂量超过21.6g·kg-1时,对肝细胞的毒性明显增强。
OBJECTIVE To study effects of serum medicated with RadixPolygoniMultifloriPreparate（RPMP）on hyperplasia and apoptosis of L02 hepatocytes.METHODS Rat models with deficiency of kidney-YIN were made using hydrocortisone,and divided into blank control group,low,moderate and high dose groups（10.8 g·kg^-1,21.6 g·kg^-1 and43.2 g·kg^-1）of RPMP.Rats were killed 7 d later to prepare medicated serum of RPMP.Morphology of L02 hepatocytes were observed by MTT,apoptosis by flow cytometry,ultrastructures by microscope.RESULTS 15% of medicated serum in high dose group could obviously inhibit hyperplasia of L02 hepatocytes treated for 48 h,and inhibition rate was 16.83%（P〈0.05）.Apoptosis rates in high and moderate dose groups were significantly increased by 19.6%（P〈0.05）and 27.8%（P〈0.01）,respectively.CONCLUSION RPMP treated rats with deficiency of kidney-YIN show obvious toxic effects on hepatocyte when RPMP dose is higher than 21.6 g·kg^-1.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2015年第18期1634-1637,共4页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学基金面上项目(编号:81373988)
关键词
制首乌
含药血清
肝细胞
凋亡
Radix Polygoni Multi flori Preparate
medicated serum
hepatocyte
apoptosis
