摘要
探讨不同浓度巴氏蘑菇多糖(ABPS)对巨噬细胞RAW264.7免疫活性的影响。以ABPS作用巨噬细胞RAW264.7 12,24 h和36 h,采用MTT法检测巨噬细胞的增殖。以ABPS及脂多糖(LPS)作用巨噬细胞RAW264.7 24 h,分别收集细胞培养上清和细胞,提取全细胞蛋白,检测iNOS含量。细胞培养上清用来检测NO生成量。结果表明:ABPS对巨噬细胞RAW264.7作用12,24 h和36 h后,与空白对照组相比,一定浓度的ABPS均可促进巨噬细胞RAW264.7的增殖,差异极显著(P<0.01)。不同浓度的ABPS和LPS作用巨噬细胞RAW264.7 24 h后,除ABPS质量浓度为250μg/m L时,iNOS生成量与阴性对照组无显著性差异外,其他浓度下iNOS及NO生成量均极显著高于阴性对照组(P<0.01)。LPS作用后iNOS和NO的生成量极显著高于ABPS组(P<0.01),其中ABPS质量浓度为1 mg/m L时,NO的生成量显著低于LPS组(P<0.05)。在一定浓度范围内,iNOS及NO生成量与ABPS浓度存在剂量效应。当ABPS质量浓度为1 mg/m L时,二者生成量最高。ABPS能够促进巨噬细胞RAW264.7的增殖及其产生iNOS和NO,对其免疫活性起正向调节作用。
To study the effects of Agaricus blazei murill polysaccharides(ABPS) on the immune activity of macrophage RAW264.7 using the cell proliferation, producing inducible nitric oxide synthase(iNO) and NO as the observation point. Using different concentrations of ABPS acting on macrophage RAW264.7 12 h, 24 h and 36 h, then we detected the macrophage proliferation by MTT. At the same time, we used different concentrations of ABPS and LPS acting on macrophage RAW264.7 for 24 h, collected the cell culture and the cells respectively, then we studied the production of NO in cell culture supernatant; collected the macrophages' whole proteins and tested the content of iNOS.The results showed that when macrophage RAW264.7 was treated with ABPS for 12 h, 24 h and 36 h, compared with the blank control group, certain concentrations of ABPS could promote the proliferation of macrophage and there were extremely significant differences(P<0.01). After macrophage RWA264.7 was treated with different concentrations of ABPS and LPS for 24 h, except the 250 μg/m L ABPS group, the production of iNO and NO were higher than those of the negative control group, which had the extremely significant differences(P <0.01). The iNO and NO production of LPS group were both significantly higher than those in the ABPS group(P<0.01). When the concentration of ABPS was 1 000μg/m L, the NO generation was lower than that of LPS group(P<0.05). The iNOS and NO contents increased with the concentration of ABPS increasing in the certain concentration range. The iNOS and NO contents were both the largest when the ABPS concentration was 1 000 μg/m L. From this study, we can conclude that ABPS could promote the proliferation of macrophage RAW264.7, and strengthen it producing iNO and NO, thus it could enhance its immune activity.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2015年第8期32-36,共5页
Journal of Chinese Institute Of Food Science and Technology
基金
山西省自然科学基金项目(2010011034-1)
山西省科技攻关项目(20130311017-3)
