摘要
目的：探讨组织多肽特异性抗原（Tissue polypeptide specific antigen，TPS）、血清癌抗原153（Cancer antigen 153，CA153）和血管内皮生长因子（Vascular endothelial growth factor，VEGF）联合检测在乳腺癌诊断和治疗中的价值。方法：1）对128例乳腺癌患者采用电化学发光法检测TPS、CA153血清水平，免疫组织化学S-P法检测VEGF的表达，另选取50例乳腺良性病变患者及30例查体健康妇女作血清学对照，分析TPS、CA153水平与VEGF在乳腺癌组中的表达及相关性；（2）三者与乳腺癌临床病理因素的关系。结果：（1）乳腺癌患者组血清TPS、CA15水平分别为269.35±101.43 U/ml、182.48±54.52 U/ml，显著高于良性对照组（95.52±22.91U/ml、19.92±3.43U/ml）和健康对照组（95.52±22.91U/ml、19.04±3.21U/ml），均P〈0.05；（2）肿瘤标记物水平高与组织学高分级、淋巴结转移及肿瘤复发等因素有关（P〈0.05），与肿瘤大小、部位、病灶数及患者年龄无关（P〉0.05）；（3）乳腺癌组VEGF表达率64.1%，明显高于良性病变组（22.0%），差异有统计学意义（P〈0.05）；（4）VEGF的过表达与乳腺癌组织学分级、淋巴结转移、远处转移有关（P〈0.05），与肿瘤大小和发病年龄无关（P〉0.05）。（6）肿瘤VEGF阳性组与阴性组组间TPS、CA153水平比较，差异有统计学意义（P〈0.05）。结论：联合检测血清中的CA153、TPS水平及组织学VEGF的表达对乳腺癌的诊断和判断预后具有价值。
Objective：To explore the clinical value of combined detection of serum tissue polypeptide specific antigen（TPS）, cancer antigen 153（CA153） and vascular endothelial growth factor（VEGF） in tissue and in the diagnosis and treatment of breast cancer. Methods：The serum from 128 patients was col ected and tumor markers of TPS and CA153 were detected, Immunhistochemical Ultra SensitiveTM S-P method was employed to detect the expression of VEGF in tissue, 50 cases of benign breast disease tissues and 30 cases of normal pregnant women were selected as a control group.Results：The serum levels of TPS and CA153 in bresat cancer patients were 269.35±101.43 U/ml and 182.48±54.52 U/ml,which were significantly higher than those in the benign breast disease （95.52±22.91U/ml, 19.92±3.43U/ml） and normal pregnant women （95.52±22.91U/ml, 19.04±3.21U/ml）, P〈0.05.The levels of the two tumor markes had a positive correlation with the grade, clinical stage, lymphnode metastasis and tumor recurrence （P〈0.05） , and negative correlation with the age and size of tumor （P〉0.05）. The positive rates of VEGF in invasive breast cancer were 64.1%, which were higher than that in benign breast disease tissues （22.0%, P〈0.05）. There was posive correlation in over-expression of VEGF with histological grade and lymph node metastasis and distant metastasis of breast cancer, and the expressions of VEGF was not related with age and size of tumor （P〉0.05）. The levels of two tumor markes TPS and CA153 had a positive correlation with the over-expression of VEGF（P〈0.05）. Conclusions：The high TPS and CA153 serum levels and over-expression of VEGF might be important biological markers for invasion and lymph node metastasis and distant metastasis of breast cancer. The dynamic combined detection of TPS and CA153 in serum and VEGF are benefit to diagnosis and interference and better prewarning markers for monitoring their recurrence and metastasis of breast cancer.
出处
《医学检验与临床》
2015年第4期28-31,共4页
Medical Laboratory Science and Clinics
基金
日照市应用技术研究与开发计划项目（2014SZSH002）,山东省医药卫生科技发展计划项目课题（2014WS0282,2014WSA11003）
关键词
乳腺癌
组织多肽特异性抗原
血清癌抗原153
血管内皮生长因子
诊断
鉴别诊断
预后
Breast neoplasms, cancer
Tissue polypeptide specific antigen
Cancer antigen 153
Vascular endothelial growth factor
Diagnosis
Differential diagnosis
Prognosis
