摘要
目的对比金标准的厚薄血膜显微镜检查法(以下简称"镜检法"),对国产疟原虫核酸实时荧光PCR检测试剂(以下简称"RT-PCR检测试剂")进行研究。方法首先提取全血样本的DNA,加入RT-PCR检测试剂进行荧光定量PCR检测,比较其相关指标。结果 2种方法经配对比较,RT-PCR检测试剂检测45份全血临床标本的总符合率达93.33%,经测序对3例不符样本进行复检,3例镜检法检测为阴性,经RT-PCR检测试剂检测为阳性的样本经测序结果显示均含有疟原虫核酸序列。结论 RT-PCR检测试剂与镜检方法在各项指标差异无统计学意义,甚至在样本处理的时效性和阳性率等方面更具有明显的优势,该实时荧光PCR试剂有良好的检测质量,特别适用于口岸、进出境等单位疟原虫DNA的检测。
Objective To compare and study new domestic parasite nucleic acid real- time fluorescent PCR rapid detection re- agent （hereinafter referred to as the RT- PCR detection reagent） and the gold standard method, thick and thin blood membrane microscopy （hereinafter referred to as the microscopy method）. Methods We extracted the DNA of whole blood clinical sam- pies, added RT- PCR detection reagent for fluorescence quantitative PCR detection, and then compared the related indicators. Results The paired comparison of the two methods showed that the total coincidence rate in 45 whole blood clinical samples was 93.33 %. The 3 disaccord samples had negative results by microscopy method and positive ones by .RT - PCR detection re- agent. Retesting demonstrated the above findings. Further sequencing revealed that the three samples contained plasmodium DNA sequences. Conclusions No statistically significant difference is found in the detected indicators between the method with RT - PCR detection reagent and the microscopy method, and the former is significantly superior to the latter in the time ef- fectiveness of sampling management, the positive rate, etc. The real- time fluorescent PCR reagent has a good detection quality, and it is especially suitable for plasmodium DNA detection in the port and entry- exit inspection and quarantine department.
出处
《实用预防医学》
CAS
2015年第10期1171-1173,1170,共4页
Practical Preventive Medicine
基金
质检总局科技计划项目(2013IK249)
