摘要
纤维品质改良是我国棉花育种的主要目标之一,纤维特异或优势表达基因的挖掘是利用基因工程手段改良纤维品质的关键。根据苏棉12纤维中优势表达的GhRACK1 EST序列设计引物,通过RACE技术克隆了GhRACK1基因的全长cDNA。推导的氨基酸序列含有4个串联的WD基序,属于WD40重复家族,与已知的RACK1蛋白同源性达70%以上,PDB模拟的蛋白三维结构也与已知的RACK1蛋白结构相似。荧光定量PCR分析表明GhRACK1在纤维中的表达量比叶片中高20倍以上。研究结果为棉花纤维品质改良基因工程提供了新的基因资源。
Fiber quality improvement is one of the main targets in cotton breeding. The discovery of specific or dominant expressed gene in fiber is the key factor that improved fiber quality by using genetic engineering strategy. In this study, we used G. hirsutum var. Sumianl2 as a starting material to clone the full-length cDNA of GhRACK1 gene by RACE techniques according known EST sequence. The deduced amino acid sequence of GhRACK1 was highly homologous to the other known RACK1 proteins and contained 4 cascade WD motifs, which belonged to WD40 family. GhRACK1 protein and known RACE1 had similar threedimensional structure by PDB simulation, qPCR results indicated that the expression level of GhRACK1 in fiber was more than 20- fold compared with leaf. This study provided new gene resource for cotton fiber improvement.
出处
《生物技术进展》
2015年第5期366-370,I0001,共6页
Current Biotechnology
基金
国家转基因生物新品种培育重大专项(2014ZX08005-003)资助
关键词
陆地棉
纤维优势表达
GhRACK1
克隆与分析
Gossypium hirsutum
fiber-superlority expression
GhRACK1
cloning and sequence analysis
